Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Progress In Microbiology and Immunology》 2001-01
Add to Favorite Get Latest Update

Investigation of factors influencing the recovery of expressed HBsAg in the host CHO C 28 cell culture

CHEN Jing yuan,GENG Yi,ZHENG Ju mei (Lanzhou Institute of Biological Products,Lanzhou 730046)  
In the process of preparation of engineering vaccine of Hepatitis B virus,the following factors were investigated:the secreting parameter of HBsAg by CHO C 28 cells line during continuous culture;the saturation degree of ammonium sulphate((NH 4) 2SO 4 to precipitate HBsAg and the storage time of CHO C 28 cell culture supernatant.The results showed influences on values of RPHA related to above three factors.It is 45% other than 42.5%(as described in the Requirement 95)that can efficiently improve the recovery rate.The storage time at 4℃ should be less than 15 days,The culture supernatant with RPHA value between 1∶32 and 1∶128 can be used as HBsAg source for further purification.
【CateGory Index】: R392-33
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 3 Hits
1 LI De-juan,CHEN Wan-ge,ZHAO Li-jian,et al. (Changchun Institute of Biological Products,Changchun 130062,PRC);Optimizing the Recombinant CHO HBsAg Purification[J];Biotechnology;2003-06
2 ZHAO Xiao-lin,JIN Li-jie,XU Dong-dong,LI De-juan,LIU Ye,GAO Song(Changchun Instititue of Biological Products,Changchun 130062,China);Validate the Capacity of Inactive Virus in Productive Technique of Recombinet Hepatitis B vaccine(CHO cell)[J];Biotechnology;2006-01
3 LU Dong,KOU Gui-ying,GOU Nin-hong,XU Feng,LIN Jie,YAO Wei(Lanzhou Institute of Biological products,Lanzhou 730046 China);The Study of Two Kinds of DMEM Medium in Culture CHO-C28 Cell[J];Pharmaceutical Biotechnology;2005-06
【Citations】
Chinese Journal Full-text Database 2 Hits
1 Wang Yan et al Changchun Institute of Biological Products, Chanchun;HBsAg Expression by CHO-C_(28) Continuous Cell Culture Using Celligen-packed Bed Basket Bioreactor and Fibra-cel Carrier[J];Chinese Journal of Biologicals;1993-04
2 Guan Guifan LiZhiqiang Wang Yan et al(Changchun Institute of Biological Products,Changchun 130062);HBsAg expression by CHO C 28 continuous cell culture applying 30 L bioreactor[J];PROGRESS IN MICROBIOLOGY AND IMMUNOLOGY;1999-04
【Co-citations】
Chinese Journal Full-text Database 5 Hits
1 Wang Yan; Luo Xuan; Liu Dawei et al (Changchun Institute of Biologcal Products,Changchun 130062);Optimum Conditions for Purification of HBsAg by Hydrophobic Interaction Chromatography[J];CHINESE JOURNAL OF BIOLOGICALS;1998-03
2 Guan Guifan LiZhiqiang Wang Yan et al(Changchun Institute of Biological Products,Changchun 130062);HBsAg expression by CHO C 28 continuous cell culture applying 30 L bioreactor[J];PROGRESS IN MICROBIOLOGY AND IMMUNOLOGY;1999-04
3 ZHANG Ying,XU Feng,ZHANG Ping,et al.(Lanzhou Institute of Biological Products,Lanzhou 730046,China);Evaluation of a new microcarrier process in bioreactor in large-scale cultivation for hepatitis B vaccine[J];Progress in Microbiology and Immunology;2007-01
4 LI Zhi-qiang,YUAN Yan-bao,ZHENG Shu-yuan,DAI Chang-hai,ZHANG Fu-kun,GU Dan-yang,QI Feng-chun (Changchun Inst.of Bio-Products,Changchun 130062);Bioreactor Application to Establish Production Technology of Freeze-Dried Rabies Vaccine (Vero Cells ) for Human Use[J];Journal of Microbiology;2010-01
5 LU Dong,KOU Gui-ying,GOU Nin-hong,XU Feng,LIN Jie,YAO Wei(Lanzhou Institute of Biological products,Lanzhou 730046 China);The Study of Two Kinds of DMEM Medium in Culture CHO-C28 Cell[J];Pharmaceutical Biotechnology;2005-06
【Co-references】
Chinese Journal Full-text Database 8 Hits
1 Mei Yafang Ren Guifang Zhu Jiming ( C.M.Chu ) ( Institute of Virology, Chinese Academy of Preventive Medicine, Beijing );PURIFICATION OF HBsAg SECRETED BY TRANS-GENIC MAMMALIAN CELL LINE[J];Chinese Journal of Virology;1990-04
2 SUN Xiang\|Ming\ ZHANG Yuan\|Xing\+* (State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237,China);Effects of Ammonia on Cell Metabolism in the Culture of Recombinant CHO Cells[J];Chinese Journal of Biotechnology;2001-03
3 Feng Xiaoli\ Jin Yetao\ Su Zhiguo\+* (Human Genome Research Center,Institute of Genetics,Chinese Academy of Sciences,Beijing\ 100101) (* State Key Laboratory of Biochemical Engineering, Institute of Chemical Metallurgy, Chinese Academy of Sciences, Beij;Instability of Proteins During Bioseparation and The Strategy For Anti-denaturation[J];PROGRESS IN BIOTECHNOLOGY;2000-03
4 Wang Yan et al Changchun Institute of Biological Products, Chanchun;HBsAg Expression by CHO-C_(28) Continuous Cell Culture Using Celligen-packed Bed Basket Bioreactor and Fibra-cel Carrier[J];Chinese Journal of Biologicals;1993-04
5 Li Bin et al(Changchun Institute of Biological Products, Changchun);Pilot Study on Purification Process of Recombinant HBsAg by Column Chromatography[J];Chinese Journal of Biologicals;1995-02
6 CHANG Yali,LIU Yan,ZHAO Yiwei,YUAN Fawen,LIANG Yan,DING Yiqing (Lanzhou Institute of Biological Products, Lanzhou 730046);Observation on Inactivation of Lipid-enveloped Viruse in Solvent/Detergent Treated Plasma[J];Journal of Microbiology;2003-06
7 LI Yu, YAO Wei, ZHANG Yan-rui, WU Yu-mei, ZHENG Ju-mei (Lan Zhou institute of biological products , Lan Zhou 730046);Effect of Metabolite on HBsAg Secretion of Recombinant CHO Cells[J];Pharmaceutical Biotechnology;2002-04
8 Xie Qingyuan (Liuzhou Biological Product Branch, Beisheng Pharmaceutical Limited-Liability Company, Liuzhou 545005, China);INFLUENCE OF S/D VIRUS INACTIVATION METHOD ON HUMAN THROMBIN ACTIVITY AND EXPERIMENTAL STUDY ON REMOVAL OF S/D[J];Chinese Journal of Disinfection;2003-03
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Wang Yan et al Changchun Institute of Biological Products, Chanchun;HBsAg Expression by CHO-C_(28) Continuous Cell Culture Using Celligen-packed Bed Basket Bioreactor and Fibra-cel Carrier[J];Chinese Journal of Biologicals;1993-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved