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《Progress In Microbiology and Immunology》 2001-01
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Analysis of amplification efficiency of PCR detection system and control of false positive contamination

FANG Yue qun,WANG Chun hui,XIE Gui lin,et al.(Lanzhou Institute of Biological Products,Lanzhou 730046)  
The experiment was to control appearance of false positive phenomenon in a nested PCR detection system by quantity indexes.The limit of cycle numbers,over which the false positive phenomenon of PCR could occur ,was firstly investigated for the given PCR detection system in the experimental environment.The amplification effciency of PCR was analyzed by regression relationship between the templates quantity and the amplification multiples of PCR,so that the detection limits of the nested PCR systems with deferent cycle numbers(C 27+15 and C 27+20 ) were determined,0.78 fg and 0.065 fg,respectively.A conception or index,false positive contamination endurance quality, which was a half of the PCR detection limit was recommended to use in detection by PCR and in this experient the indexes were 0.39 fg(C 27+15 ) and 0.033 fg(C 27+20 ) respectively.
【CateGory Index】: R346
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