Establishment of detection method on BHK _(21 ) cell for neutralising antibody to Hanta virus(HV)
WANG Xiao yu,FU Zeng wu,Hui Lian,et al.(Changchun Institute of Biological Products,Changchun,130062)
In this report eight strains of HV,including type 1.76,3205A118,Chen,J 3,J 5 and J 12 ;type Ⅱ.UR,R 22 and L 99 ,which are cultured and observed on BHK 21 cells and the regular cytopathic effect has been found on BHK 21 cells as the result of the virus infection,the virus infective titer then could be deteced.By PRNT,the titer of antibodies to different HV strains on BHK 21 are consistent with that on Vero 6 cells and BHK 21 cells have the higher stability and specificity.So the BHK 21 cell could be used as the alternative sensitive cell line in detection of HV neutralising antibody and study some biological properties of HV.And this line is practical to associated methodology.
【CateGory Index】： R446.62