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《Progress in Microbiology and Immunology》 2006-01
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Preparation of high titer antiserum for mumps virus

LIU Song-you,JIN Xiao,LI Shu-yun,et al.(Beijing Tiantan Biological Products Co.Ltd.,Beijing 100024,China)  
Preparation of high titer antisera for mumps virus identification and virus neutralization test for quality control in the vaccine production.SPF chickens were immunized by mumps virus propagated in embryonating eggs from a SPF flock and rabbits immunized by the virus propagated in Vero cells,concentrated by PEG precipitation and purified by ultra centrifugation.Both sera were compared,the cumulative titer of antisera from SPF chikens for neutralization antibodies was 1: 1716 in GMT,the antisera from,rabbits had a result of 1:732 in GMT.Both sera are suitable for qualities control of the mumps vaccine either monovalence or multiplevalence as in MMR or measles-rubella combined vaccine.The antisera prepared from SPF chickens have been free from antibodies contamination to related pathogens,it is essential for quality control of vaccine.The antisera are easy to prepare and the result could be directly proved.
【CateGory Index】: R392
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