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《Progress in Microbiology and Immunology》 2007-01
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Evaluation of a new microcarrier process in bioreactor in large-scale cultivation for hepatitis B vaccine

ZHANG Ying,XU Feng,ZHANG Ping,et al.(Lanzhou Institute of Biological Products,Lanzhou 730046,China)  
Bioreactor process for recombinant CHO-C28 cell cultivation of hepatitis B vaccine was investigated in this study.A piece of three dimensional polyester fiber discs was used as support material and a continuous perfusion mode was used for cultivation.The culture conditions such as inoculation of cell density,pH,DO and perfusion velocity were studied respectively.The continuous three run cultivations were performed,each run maintained for 60 days.The optimal culture conditions are in following pH 6.80~7.10,DO 20%~30%,temperature 36~37℃,perfusion velocity 138ml/h and inoculation cell density 1.9 106 cell/ml.The ultimate average titer for harvested culture is 1∶256 in RPHA,and the content of purified HBsAg is 0.912mg/L.Finally,the advantages and disadvantages for both culture systems of roller bottle and bioreactor were compared and summarized.
【CateGory Index】: R392
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Chinese Journal Full-text Database 1 Hits
1 Guan Guifan LiZhiqiang Wang Yan et al(Changchun Institute of Biological Products,Changchun 130062);HBsAg expression by CHO C 28 continuous cell culture applying 30 L bioreactor[J];PROGRESS IN MICROBIOLOGY AND IMMUNOLOGY;1999-04
Chinese Journal Full-text Database 3 Hits
1 BAI Li,ZHANG Shu-xiang,TANG Yin,GUO Mei-jin,ZHANG Si-liang(State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,China);Performance of the Frusto-Conical Bottom Bioreactor in Mammalian Cell Suspension Culture[J];Journal of East China University of Science and Technology(Natural Science Edition);2008-03
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