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《Progress in Microbiology and Immunology》 2007-01
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Investigation on using S79 strain mumps virus in development of diagnostic reagent

ZHANG Guo-qiang1,LI Xiao-qian2,ZHAO Kai2(1 Beijing Tiantan Biological Products CO.LTD,Beijing 100024,China;2 National Vaccine & Serum Institute,Beijing 100024,China)  
The purified antigen of mumps virus S-79strain was used to develop ELISA diagnostic reagent in detection of antibody(IgG) to mumps virus.The tissue culture of mumps virus S79 strain was purified by ultrafiltration on hollow fiber,PEG precipitation and density gradient ultracentrifugation.The purified mumps virus was used as a coating antigen in ELISA reagents,and the ELISA kit was compared with SIGMA kit.The results showed that the ratio of specific activity in purified mumps virus was 412.9HAU/mg by hemagglutination test,and the rate of non-specific protein removed was 99.5%.In comparison with SIGMA kit,the sensitivity of developed ELISA kit was 94.4%,the specificity was 91.7% and conformity was 93.3%.It indicated that the purified mumps virus from S79 strain can be used as coating antigen in preparation of mumps ELISA kit in a large scale.
【CateGory Index】: R392
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 Yan Youwang, Zhang Jiakai,Shu Sixing, et alEpidemic Prevention Station of Jingzhou District, Jingzhou city, 434100, Hubei Province;Evaluation on the Epidemilogical Effect of S79 Live Attenuated Mumps Vaccine[J];中国计划免疫;1998-03
【Co-citations】
Chinese Journal Full-text Database 4 Hits
1 GAO Jun,MA Fu-bao,ZHANG Long-hua,et al.(Jiangsu Provincial Center for Disease Prevention and Control,Nanjing 210009,China);STUDY ON THE EPIDEMIOLOGICAL EFFECT OF LIVE ATTENUATED MUMPS VACCINE[J];现代预防医学;2010-22
2 ZHANG Guo-qiang1,LI Xiao-qian2,ZHAO Kai2(1 Beijing Tiantan Biological Products CO.LTD,Beijing 100024,China;2 National Vaccine & Serum Institute,Beijing 100024,China);Investigation on using S79 strain mumps virus in development of diagnostic reagent[J];微生物学免疫学进展;2007-01
3 Chen Zhi-hui.(Shanghai Institute of Biological Products, Shanghai 200052, China.);Mumps Virus and the Vaccines[J];中国计划免疫;2004-02
4 LIU Pei-ning,DU Ji-mei,TANG Chun-ping,et al.;PERSISTANCE OF ANTIBODY 3 YEARS AFTER FIRST VACCINATION WITH SINGALVALENT RUBELLA AND MUMPS VACCINE.[J];现代预防医学;2002-03
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Guo Shaohong Xu Tianqiang Wu Weishou Dong ChunmingWang Gangyi Xu Qi Shi Yan Chen Haiping Ze Wenyuan;THE IMMUNE RESPONSES AND SYSTEMIC REACTIONAFTER VACCINATION WTTH S79 LIVE ATTENUATEDLYOPHILIZED MUMPS VACCINE IN CHILDREN[J];中国计划免疫;1997-01
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