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Detecting the function of B memory cell in PS1 gene mutant knock in mice model

JIA Wei-hong 1,2,DUAN Yue-qiang2,LI Xiao-nan2,et al.(1 Department of Respiration,Xijing Hospital;Fourth Military Medical University,Xi an 710032,China;2 Institute of Microbiology and Epidemiology,Academy of Military Sciences,Beijing 100071 China)  
To detect the function of B memory cell in PS1 gene mutation knock in mice model,ELISA method was used for antibody assay in different timepoints for maintenance of immunological memory observation;isolating spleen cell and peripheral blood lymphocyte after boosting one time with OVA,testing the function of lymphocyte both in central and peripheral immune system using lymphocyte proliferation assay and flow cytometry(FCM) methods separately.The mice immunized with vSC8 vaccine were challenged using WR,to observe the survival rate of mice in different groups.Lymphocyte proliferation assay and FCM showed that there are the difference between groups(P0.0001,=0.045);vaccinia immunized mice challenged by WR strain one year later,the C57BL/6 mice showed better survival condition than those of PS1 gene mutation knock in mice(P0.0001).So PS1 gene may play a key role in B cell,including B memory cell function.The mechanism which was involved in T cell function or influence B cell function directly still need to be quested for in the near future.
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