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Monitoring the process optimization of an acellular pertussis vaccine by a sandwich ELISA

CHEN Jing,JIANG Qiang-hua,XIONG Xiao-pei,et al.(Wuhan Institute of Biologic Products,430060,China)  
After optimizing the process of acellular pertussis vaccine(APV),Two Sandwish ELISA methods were used to detect the effective component content of APV.The results of ELISA were validated with those of potency test.All of results of this two methods meet is in line with the standard requirement of "The Chinese Pharmacopoeia,Section Ⅲ,2005".Since the Sandwith ELISA method are of the excellent specificity and sensitivity,this method can be applied to the quality control of APV.
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