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《Progress in Microbiology and Immunology》 2011-01
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High expression of rhAPC in serum free-medium by immobilized HEK cells

Li Zheng,Zeng Xian-wu(Chengdu Institute of Biological Products,Chengdu 610063,China)  
The cultivation of immobilized HEK cells was performed and the expression level of rhAPC was detected.The adhere cells were adapted for suspension cultivation in serum-free medium,then these cells were immobilized with calcium alginate and cultured in serum-free medium.The results showed that rhAPC expression level of the immobilized cells is higher than that of non-immobilized cells.The highest expression level is 50mg/L,the high density culture of immobilized cells can be achieved.
【CateGory Index】: R329
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