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A HPLC method for the determination the content of lactose in group A and C meningococcal vaccine

LUO Li-hong,FENG Xiao,WEI Ran,et al.(Lanzhou Institute of Biological Products,Lanzhou 730046,China)  
In this paper,a high-performance liquid chromatography(HPLC) method for determination the content of lactose in group A and C meningococcal vaccine was established.First,the meningococcal polysaccharides in sample were removed by centrifugation.Then,the supernatants were separated on an cation-exchange column and detected with a refractive index detector(RID).Finally,the calibration curves were established by external standard method.The relative coefficient for linearity of the method is greater than 0.9999,the recovery rate of the lactose is 98.8%.The coefficient of variation(CV)is 1.2%.The HPLC method is accurate and repetition.It can be used as a rapid means for quantitative determination of the lactose in group A and C meningococcal vaccine.
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