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《Progress in Microbiology and Immunology》 2014-01
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Expression,purification,assymbly and identification of virus like particle antigens

YU Yong-li;Department of Immunolog,School of Basic Medical Sciences,Jilin University;  
Virus-like particles( VLPs) are particles formed by one or several structural proteins from viruses,with similar outside structure and antigenicity of the native viruses,containing no virus genome. Three VLP based human vaccines have been licensed for preventing infections of hepatitis B virus,human papilloma virus and hepatitis E virus,respectively. VLP antigen can be produced in bacteria,yeasts,insect cells,mammalian cells and plant cells. The produced VLP are purified through four stepes including lysis,extraction,concentration and refinement. The potency and stability of the VLP antigens could be improved by treatment of disassembl / reassembly. The related detections have been performed to monitor the identity,quality,potency and purity of the VLP antigens in the process of development and mamufacture.
【CateGory Index】: R392
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