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《Progress in Microbiology and Immunology》 2014-01
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Expression,purification,assymbly and identification of virus like particle antigens

YU Yong-li;Department of Immunolog,School of Basic Medical Sciences,Jilin University;  
Virus-like particles( VLPs) are particles formed by one or several structural proteins from viruses,with similar outside structure and antigenicity of the native viruses,containing no virus genome. Three VLP based human vaccines have been licensed for preventing infections of hepatitis B virus,human papilloma virus and hepatitis E virus,respectively. VLP antigen can be produced in bacteria,yeasts,insect cells,mammalian cells and plant cells. The produced VLP are purified through four stepes including lysis,extraction,concentration and refinement. The potency and stability of the VLP antigens could be improved by treatment of disassembl / reassembly. The related detections have been performed to monitor the identity,quality,potency and purity of the VLP antigens in the process of development and mamufacture.
【CateGory Index】: R392
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【References】
Chinese Journal Full-text Database 1 Hits
1 BAI Liang;CHENG Yan;CAO Rui-zhen;College of Life Science, Inner Mongolia University for Nationalities;;Progress in research on virus-like particles of hepatitis B virus core protein as a vaccine carrier[J];中国生物制品学杂志;2015-12
【Co-references】
Chinese Journal Full-text Database 4 Hits
1 BAI Liang;CHENG Yan;XU Fangfei;LIU Yan;ZHANG Shujun;CAO Ruizhen;Department of Biochemistry and Molecular Biology,School of Life Science,Inner Mongolia University for Nationalities;Department of Immunology and Microbiology,School of Medical Sciences,Inner Mongolia University for Nationalities;Ji'an Yisheng Pharmaceutical Co.Ltd.,Jilin Provice;;Fusion expression of Staphylococcus aureus IsdB epitope protein and HBc protein[J];吉林大学学报(医学版);2015-02
2 TONG Yan-jun;XIE Zhen;JIANG Lin;Lanzhou Institute of Biological Products Co. Ltd,Center for Gansu Provincial Vaccine Engineering Research;;The virus-like particles and its vaccine research[J];微生物学免疫学进展;2015-04
3 YU Yong-li;Department of Immunolog,School of Basic Medical Sciences,Jilin University;;Expression,purification,assymbly and identification of virus like particle antigens[J];微生物学免疫学进展;2014-01
4 HAN Jun-Feng (Institute of Immunology, PLA , Third Military Medical University, Chongqing 400038, China);HBcAg virus like particles and vaccine design[J];免疫学杂志;2005-S1
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