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《Progress in Microbiology and Immunology》 2014-01
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Analysis for the differences of macrophages U937 apoptosis infected by clinical isolates of Mycobacterium tuberculosis with different resistant phenotype

HU Zu-qiong;TAN Yao-ju;GUAN Pin;CHEN Hong;MENG Fan-rong;HUANG Yan-jun;LIU Yan-wen;Guangzhou chest hospital;  
Objective To compare the apoptosis rate of macrophages U937 infected with clinical isolates of MDR-TB and susceptible Mycobacterium tuberculosis by using a flow cytometry. Methods Macrophages U937 were respectively infected with MDR-TB and susceptible clinical isolates,then the apoptosis rate of macrophages U937 were detected by the flow cytometry after infection in 2,4,8 and 12 hs and the phase changes of apoptosis rate of infected macrophages U937 were analyzed. Results U937 apoptosis can be induced both by the MDR-TB and the susceptible isolates,but the differences are significant in the apoptosis rates in between two types of isolates( P0.01). The apoptosis rates of U937 infected with MDRTB are significantly lower than that with susceptible isolates,and all of the apoptosis rates of U937 after infection in 2,4, 8 and 12 hs with MDR-TB are significantly lower than that with susceptible isolates. The apoptosis rates of U937 increase markedly in early stage of infection,especially after infection in 2 hs. The apoptosis rates slowly rise down after infection in 4 hs. Conclusion The macrophage U937 apoptosis rapidly occurrs after infecting with the isolates of mycobacterium tuberculosis. The apoptosis rates of U937 are correlated to the isolates of MDR-TB,and are significantly higher in susceptible isolates than these in MDR-TB isolates,but rise slowly down after infection in 4 hs.
【Fund】: 广州市医药卫生科技项目(201102A213131)
【CateGory Index】: R378.911
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【References】
Chinese Journal Full-text Database 1 Hits
1 ZHAO Hongmei;ZHANG Fang;Department of Internal Medicine-Tuberculosis,China Shenyang Chest Hospital;;Significance of Mycobacterium Tuberculosis-Specific Antigens Detection in the Clinical Diagnosis[J];中国卫生标准管理;2015-01
【Citations】
Chinese Journal Full-text Database 2 Hits
1 DONG Wei-Jie,LIU Dan-Xia,LI Wei,LIU Yun-Xia,TUO Qing-Zhang,WU Fang,ZHANG Le,ZHANG Wan-Jiang.Department of Microbiology Immunology,Medical School,Shihezi University/Laboratory of Xinjiang Endemic and Ethnic Diseases,Shihezi University,Shihezi 832002,China;The influence of theapoptosis rate and the expression of caspase-3 in the macrophages infected by Mycobacterium tuberculosis[J];中国免疫学杂志;2013-04
2 ZHANG Feng,XU Xiaoyong,SUN Wenkui,SHI Yi,LI Ziling.(Department of Respiratory Medicine, The Second Military Medical University/Nanjing General Hospital of Nanjing Military Command, PLA,Nanjing 210002,China);The role of Toll-like receptor 2 in Mycobacterium-induced macrophage apoptosis[J];中国感染与化疗杂志;2011-01
【Co-citations】
Chinese Journal Full-text Database 7 Hits
1 Zhang Qiquan;Hu Xianwen;Uuan Xiaowen;Wang Jingxian;He Shufang;Zhang Ye;Department of Anesthesiology,Second Affiliated Hospital of Anhui Medical University;;Effect of sevoflurane postconditioning on inositol-requiring enzyme 1 signaling pathway in brain tissues in a rat model of hemorrhagic shock and resuscitation[J];中华麻醉学杂志;2017-06
2 SHEN Hong-wen;LI Xiao-fang;XU Xiao-yi;WU Xu-min;CHEN Gang;Quzhou City Chinese Medicine Hospital;;Application value of ultrasound in the diagnosis of patients with peritonitis caused by Mycobacterium tuberculosis[J];中华医院感染学杂志;2015-19
3 ZHU Kunsheng;YI Min;ZHOU Xiangdong;LI Shengjin;Department of Respiratory Diseases, Second Affiliated Hospital, Chongqing Medical University;;Effect of Mycobacterium tuberculosis eis gene on cytokines production of THP-1 cell[J];免疫学杂志;2015-08
4 WANG Chan;WANG Xin-min;WANG Fei-yu;ZHANG Yu-qing;CAO Xu-dong;WU Jiang-dong;WU Fang;ZHANG Wan-jiang;ZHANG Le;Medical College of Shihezi University Department of Pathogen Biology and Immunology;Medical College of Shihezi University Key Laboratory of Endemic and Ethnic Diseases in Xinjiang of Education Ministry;Medical College of Shihezi University Department of Urinary Surgery, the First Affiliated Hospital;Medical College of Shihezi University Department of Pathophysiology;;Construction and identification of Mcl-1 gene shRNA expression plasmid in targeted silenced macrophages[J];西安交通大学学报(医学版);2015-04
5 WANG Chan;WANG Xin-Min;WANG Fei-Yu;ZHANG Yu-Qing;CAO Xu-Dong;WU Jiang-Dong;WU Fang;ZHANG Wan-Jiang;ZHANG Le;Department of Microbiology Immunology,Department of Pathophysiology Medical School,Shihezi University/Laboratory of Xinjiang Endemic and Ethnic Diseases,Shihezi University;;Specificity of screening of short hairpin RNA targeting Mcl-1 gene in Raw264.7 cells[J];中国免疫学杂志;2015-02
6 MENG Fanrong;XIE Bei;LIU Zhihui;The Pulmonary Disease Research Institute of Guangzhou Chest Hospital;;RESEARCH PROGRESS ON IMMUNE RESPONSE OF MACROPHAGE TO MYCOBACTERIUM TUBERCULOSIS[J];现代医院;2013-12
7 HU Zu-qiong;TAN Yao-ju;GUAN Pin;CHEN Hong;MENG Fan-rong;HUANG Yan-jun;LIU Yan-wen;Guangzhou chest hospital;;Analysis for the differences of macrophages U937 apoptosis infected by clinical isolates of Mycobacterium tuberculosis with different resistant phenotype[J];微生物学免疫学进展;2014-01
【Co-references】
Chinese Journal Full-text Database 4 Hits
1 HU Zu-qiong;TAN Yao-ju;GUAN Pin;CHEN Hong;MENG Fan-rong;HUANG Yan-jun;LIU Yan-wen;Guangzhou chest hospital;;Analysis for the differences of macrophages U937 apoptosis infected by clinical isolates of Mycobacterium tuberculosis with different resistant phenotype[J];微生物学免疫学进展;2014-01
2 CHE Nan-ying, DING Zhi-xin, WANG Wei, et al (Department of Bacteriology and Immunology, Beijing Chest Hospital, Capital Medical University ,Beijing Tuberculosis and Thoracic Tumors Research Institute, Beijing 101149);Research on immunodiagnostic value of specific genes of fusion protein MPT64-Rv1985c in Mycobacterium tuberculosis[J];北京医学;2012-09
3 ZHANG Jun-xian,WU Xue-qiong~*,ZHANG Cui-ying,DONG Mei (Army Tuberculosis Prevention and Control Key Laboratory,Institute for Tuberculosis Research, the 309th Hospital of PI A,Beijing 100091.China);SERODIAGNOSIS OF RECOMBINANT Ag85A PROTEIN FROM MYCOBACTERIUM TUBERCULOSIS[J];河北医科大学学报;2011-06
4 SHI Jun-fan~*,SONG Guang-zhong,PAN Jie-chao,LOU Lei-jun,YANG Ming-jin,LI Zhao-dong.* Institute of Parasitic Diseases,Zhejiang Academy of Medical Sciences,Hangzhou 310013,China;Production of recombinant fusion protein rESAT-6-CFP-10 and its application in tuberculosis serodiagnosis[J];国际流行病学传染病学杂志;2010-06
【Secondary References】
Chinese Journal Full-text Database 2 Hits
1 WANG Lin;Third People's Hospital of Kunming City(Kunming Tuberculosis Prevention and Control Center);;The Diagnostic Value of Mycobacterium Tuberculosis Specific Antigen ESAT-6 and CFP-10 in Patients With Pulmonary Tuberculosis and Pneumonia[J];中国卫生标准管理;2015-32
2 TAN Yingzheng;CHEN Shuanghua;LONG Yunzhu;LI Dan;FU Jingli;ZHOU Dujuan;FU Jinqiu;Department of Infectious Diseases, Zhuzhou Central Hospital,Hu'nan Province;Hu'nan Traditional Chinese Medicine College;;Evaluation on the diagnostic value of Xpert MTB/RIF test towards smear negative plumonary tuberculosis[J];中国医药导报;2015-29
【Secondary Citations】
Chinese Journal Full-text Database 3 Hits
1 YAO Nan,DONG Jiang-tao,XU Fang,TIAN Xi-ze,WU Fang,ZHANG Le,ZHANG Wan-jiang(Department of Pathophysiology,Medical School,Laboratory of Xinjiang Endemic and Ethnic Diseases,Shihezi University,Shihezi 832002,Xinjiang,China);Apoptosis rate of and changes over time in macrophages infected with Mycobacterium tuberculosis with different levels of virulence tested with flow cytometry[J];中国病原生物学杂志;2012-02
2 LI Zi-ling1,ZHANG Xin-hua2,ZHANG Fang1,SHAO Hai-feng3,WANG Cui-hong1,ZHANG Peng-peng1(1.Department of Respiratory;2.Department of Pathology;3.Institute of Medical Laboratory Science of PLA,Nanjing General Hospital of Nanjing Command,PLA,Nanjing 210002,Jiangsu,China);A primary survey of the histopathological characteristic in nontuberculous mycobacteria lymphadenitis[J];医学研究生学报;2007-02
3 LI Chuan-you, XING Ai-ying, LI Liang, WEI Pan-jian,GU Shu-xiang, CHEN Xiao-you, DUAN Hong-fei, CHEN Xi, XIE Li, MA Yu. Beijing Tuberculosis & Thoracic Tumor Research Institute,Beijing 101149,China;Nitric oxide production and expression of cytokines by macrophages infected by M. tuberculosis H_(37)R_v[J];中华结核和呼吸杂志;2003-04
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