The replacement of main materials for culture of Clostridium.tetani in a toxin production medium
CAO Ling;YANG Hai-zhen;SUN Yi-mei;LI Guo-yan;XIE Peng;Lanzhou Institute of Biological Products Co.,Ltd.,Center for Gansu Provincial Vaccine Engineering Research;
Objective To explore using dry powdered liver extract powder,trypticase,and peptone as main materials,it is the need for large-scale production,preparation was carried out for the medium production of tetanus toxin instead o f the traditional medium contained liver extracellular fluid,trypsin digestion of casein and concentrated peptone water. M ethods The prepared midea and the traditional media were both used in production process of tetanus toxin,from a simple procedure to a large scale gradually,different components of the culture medium were changed by the multi-batch experiments and inoculate bacterial seeds,and cultured toxin production levels were obtained and compared,so did for index of biochemical standard. Firstly,on the basis of the replacement of liver extract powder to the extra dialysis fluid,then selection peptone to replace concentrated peptone water was carried out. In combination of these three materials in a toxin production medium,comparative tests were performed in both of media. Results Several tests are completed,from a small capacity to a production process,the biochemistry indexes are obtained in media after cultivation of bacteria. In preparation of toxin,the medium contained liver extract powder,trypticase and peptone was compared to the traditional medium,it has no statistical significant difference( P0.05) in both media. Conclusions The liver extract powder,trypticase,and peptone can replace the liver extra-dialysis fluid,trypsin digestion of casein and concentrated peptone water in the traditional medium in preparation of tetanus toxin in an industrial production.
【CateGory Index】： R378.81