Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Microbiologica Sinica》 1984-01
Add to Favorite Get Latest Update

TRANSFECTION OF RNA FROM μ_2 RNA PHAGE

Zhang Jiren;Chen Shen Institute of Biophysics, Academia Sinica Beijing  
Three methods: 1) Ca~(2+) treatment, 2)hypertonic medium, and 3) lysozyme-EDTAsystem in the presence of protamine sulfatewere applied to compare the efficiency oftransfection of purified μ_2 RNA. Amongthem, the third method is the most effec-tive for transfection with plaque formingunits (p. f. u) reaching to 2.1×10~6 per μgRNA. The action of protamine sulfate andother influencing factors on the transfectionof μ_2 RNA, such as the concentration ofRNA, the partial digestion of the RNA mo-lecule in 5' or 3' terminal ends, the integra-tion of ethdium bromide and actinomycinD, and the heat denaturation were also ob-served and discussed in detail.
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved