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《Acta Microbiologica Sinica》 1984-01
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Qiu Xiubao;Cheng Xiulan Institute of Microbiology, Academia Sinica, Beijing  
An alkaline proteinase produced by Ba-cillus pumilus was partially purified througha process involving ammonium sulfate pre-ciptation, Sephadex G-25 filtration, chroma-tography on DEAE-cellulose and lyophyliza-tion, and a final product, a white powderwas obtained. The specific activity of en-zyme preparation after purification increased2.6 times. The optimum pH and tempera-ture for casein hydrolysis were 9.0 and 50℃,respectively. The enzyme was stable overthe pH range from 6 to 10. Ca~(2+) enhancedthe heat stability of the enzyme. The enzyme was completely inactivitedafter treatment with DFP (2.5 mM ) or NBS(1×10~(-3)M), but not with PCMB and O-PTH. Theccation such as Cu~(2+), Fe~(2+) andHg~+ (1×10~3-M ) inhibited the enzyme ac-tivity, while Mn~(2+) trends to stimulate acti-vity. Casein, haemoglobin, snake venom pro-tein and fibrin were susceptible to enzymatichydrolysis.
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Chinese Journal Full-text Database 2 Hits
1 SUN Mi 1 XIU Zhao yang 2 WANG Yue jun 1 CHEN Li fang 3 ZHANG Yun bo 1 HONG Yi guo 4 YAN Xiao ling 1 HAN Yan bo 4 LI Jing 1 CHEN Liang 4 ( 1Open Laboratory of Marine Enzyme & Enzyme Engineering, Yellow Sea Fisheries Researc;Study on properties of low-temperature protease of Flavobacterium YS-9412-130[J];Marine Fisherries Reseach;2001-02
2 YING Sheng-Hua;FENG Ming-Guang(Dopartment of Biological Sciences,Zhejiang University (Huajiachi Campus), Hangzhou 310029);ACTIVITY OF ALKALINE PROTEASE PRODUCED BYCONIDIOBOLUS CORONATUS IN LIQUID CULTURE[J];MYCOSYSTEMA;2000-01
Chinese Journal Full-text Database 3 Hits
1 Huang Fan, Li Xinzhi, Zhang Yu, Tang Maohong, Zhang Yinmei (Institute of Genetics, Aeademia Sinica, Beijing 100101);Construction of Engineering Strain with High Production of Oxidatively Resistant Subtilisin I. Purification and Characterization of Oxidatively Resistant Subtilisin from Bacillus subtilis B135[J];Industrial Microbiology;1996-03
2 Qiu Xiubao (Institute of Microbiology, Academia Sinica, Beijing 100080) Gao Dong Wang Yingda (Department of Microbiology, Shandong University, Jinan 250100);STUDIES ON PURIFICATION AND SOME PROPERTIES OF PROTEASE BP FROM BACILLUS PUM1LUS[J];Acta Microbiologica Sinica;1994-04
3 Feng Qingping Xue Lingui(Department of Biology, Lanzhou University, Lanzhou 730000);THE STUDY OF SELECTION FOR HIGH-YIELD THERMOSTABLE ALKALINE PROTEASE PRODUCING STRAIN AFTER COMPLEX MUTAGEMISIS OF PROTOPLASTS[J];Acta Microbiologica Sinica;1996-06
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 Deng Kaiye~(1,2),Li Yu~1,Ren LuQuan~2 (1.Jilin Agricultural University,Changchun1301182.The Key Laboratory for Terrain Machine Bionics Engineering,Ministry of Education,Changchun,Jilin 130051);The research progress of fugus based enzyme products[J];Quarterly of Forest By-product and Speciality In China;2005-06
2 DU Jun-ling~1,CUI Yan~1,HAN Ji-gang~1,ZHAO Gang-yong~2, GU Ya-jun~2,ZHU Bao-cheng~(1,3) (1. College of Life Science, Hebei University, Baoding 071002, China; 2. Microbiology Institute of Hebei Province Academy of Sciences, Baoding 071051, China; 3. College of Life Science, Agricultrual University of Hebei, Baoding 071001, China);Purification and characterization of the denude alkaline protease[J];Journal of Agricultural University of Hebei;2004-03
3 LIN Bai-xue,HUANG Zhi-qiang,XIE Lian-hui (Key Laboratory of Biopesticide and Chemical Biology of Ministry of Education;Institute of Plant Virology,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China);Enzyme properties of alkaline protease produced by marine bacterium strain 15E (Brevibacterium oxydans)[J];Journal of Fujian Agriculture and Forestry University(Natural Science Edition);2008-02
4 HU Xue-zhi1, WANG Jun2(1.Shanghai Institute of Industrial Microbiology,Shanghai 200233;2.Shanghai Research Center of Biotechnology,Chinese Academy of Science,Shanghai 200233);Advances in protease production and its applications[J];Industrial Microbiology;2008-04
5 WANG Yujing 1, ZHANG Manping 1, SUN Mi 2, WANG Yuejun 2,XIE Hongguo 3, CHEN Chunguang 3(1. College of Chemistry and Chemical Engineering, Ocean Universityof China, Qingdao 266003;2. Open Laboratory of Mmarine Eenzyme & Enzyme Eengineering,Yellow Sea Fisheries Research Institute,Qingdao 266071;3. Qingdao University of Science and Technology, Qingdao 266042);THE CHEMICAL CHARACTERISTICS OF MARINE LOW-TEMPERATURE ALKALINE PROTEASE(YS-80-122)[J];Transaction of Oceanology and Limnology;2004-01
6 HONG Yi guo SUN Mi XIU Zhao yang * ZHANG Yun bo WANG Hai ying WANG Yue jun (Yellow Sea Fisheries Research Institute, Qingdao 266071);Analysis of functional essential groups of marine low-temperature alkaline protease from Flavobacterium yellowsea YS-9412-130[J];Marine Fisherries Reseach;2002-03
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9 HU Xue-zhi(Bioengineering Research Center,Chinese Academy of Sciences,Shanghai,200000);The production and application of protease[J];Jiangsu Condiment and Subsidiary Food;2007-04
10 Yang Shengyuan Lu Zhaoxin(College of Food Science and Technology,Nanjing Agriculture University,Nanjing,210095);Research Progress on Microbial Low-temperature Alkaline Protease[J];Food and Fermentation Industries;2004-04
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