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《Acta Microbiologica Sinica》 1984-01
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STUDY ON INTERSPECIFIC PROTOPLAST FUSION BETWEEN BACILLUS SUBTILIS AND BACILLUS LICHENIFORMIS

Zhu Gejian;Zhou Lipin;Wang Ping;Tang Bin Wuxi College of Light Industry, Wuxi  
Interspecific fusants between Bacillussubtilis AS 1.398-28-6 and Bacillus licheni-formis AS 1.807-9-9 have been obtained. Thestrain AS 1.398 produces neutral proteinaseand the strain AS 1.807 produces alkalineproteinase. It is better to prepare the protop-last by using lysozyme 2500 u/ml. pH 6.7-7.0 in SMM or NSM at 35℃. The rates ofprotoplast formation and protoplast regene-ration were 99.9% and 1-4%, respecti-vely. The fusion was carried out in SMMor NSM containing 30% polyethene glycol(MW 6000), and the fusion frequency isabout 7.47×10~(-4)-2.28×10~(-4). These fusants were allowed to growfor 4 generations on selective medium inorder to examine their stability and 12-20% of them were stable. The morphologyof cells and colonies were different fromtheir parents ones. The production levelof some fusants wns 30% higher. than thatof the parent strain.
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