STUDIES ON THE CONDITIONS FOR CYCLODEXTRIN-GLUCOSYL TRANSFERASE PRODUCTION BY TWO TYPES OF ALKALIPHILIC BACTERIA AND FOR ENZYMIC REACTION
Dan Jialin;Wang Shizhu;Xu Guanzu;Xu Chunxi Institute of Microbiology, Acedemia Sinica, Beijing
Cyclodextrin-glucosyl-transferase wasproduced by two types of alkaliphilic bac-teria, a strain of spore-forming bacteria and astrain of non-spore-forming bacteria. Yeastextract and corn steep liquor were the bestnutrients for enzyme production by thestrains of spore-forming and non-spore for-ming bacteria respectively. The suitable car-bon and nitrogen sources for enzyme pro-duction were confirmed. There was no signi-ficant difference in the conditions for enzy-mic reaction in these two cultures. Beta-cy-clodextrin was the main product of theenzyme reaction. The discovery of cyclodex-trin-glucosyl-transferase produced by non-spore-forming bacteria enlarged the scopefor searching this enzyme.