Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Microbiologica Sinica》 2003-01
Add to Favorite Get Latest Update

Identification and Functional Characterization of sawE—a Gene Involved in Differentiation of Streptomyces ansochromogenes 7100

Xu Jianyong Liu Gang Tan Huarong ** (Institute of Microbiology, Chinese Academy of Science, Beijing 100080, China)  
A 2.8kb DNA fragment including whiB-like gene was cloned from the partial DNA library of Streptomyces ansochromogenes 7100 by using whiB of Streptomyces coelicolor J1501 as a probe. The nucleotide sequence of 1.4kb DNA fragment was determined and analyzed. The result showed that the fragment contains one complete open reading frame-designated sawE. sawE shows strong identity to whiB, an essential gene for early stage of sporulation. The deduced product of sawE gene is a regulatory protein. sawE disruptant was blocked at aerial hyphae stage of Streptomyces ansochromogenes 7100, no sporulation septation, no formation of grey mature spore. Disruptants maintained white phenotype on prolonged incubation. The result indicated that sawE is essential for the morphological differentiation of S. ansochromogenes.
【Fund】: 国家自然科学基金重点资助项目 ( 39830 0 1 0 )~~
【CateGory Index】: Q933
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 1 Hits
1 Tan Huarong Wu Wei Tian Yuqing Yang Haihua Song Youxin Dong Kening Huang Xiping Song Dakang (Institute of Microbiology, Academia Sinica, Beijing 100080);STUDIES ON THE DIFFERENTIATION AND PROPERTIES OF STREPTOMYCES ANSOCHROMOGENES[J];Acta Microbiologica Sinica;1994-05
【Co-citations】
Chinese Journal Full-text Database 4 Hits
1 Tan Huarong Xu Chong Tian Yuqing Xu Jianyong (Institute of microbiology, Academia Sinica,Beijing 100080) Chater K F (John Innes Institute, Norwich, England);THE EFFECT OF A PROMOTER P_(28-1) OF BACILLUS SUBTILIS ON STREPTOMYCES DIFFERENTIATION[J];Acta Microbiologica Sinica;1994-05
2 Tan Huarong Wu Wei Tian Yuqing Yang Haihua Song Youxin Dong Kening Huang Xiping Song Dakang (Institute of Microbiology, Academia Sinica, Beijing 100080);STUDIES ON THE DIFFERENTIATION AND PROPERTIES OF STREPTOMYCES ANSOCHROMOGENES[J];Acta Microbiologica Sinica;1994-05
3 YANG Yan-ling YANG Hai-hua * TAN Hua-rong (Institute of Microbiology, Chinese Academy of Siences, Beijing 100080, China);Preliminary study on autoregulation of samR involved in development and differentiation of Streptomyces ansochromogenes[J];Acta Microbiologica Sinica;2005-01
4 LEI Jian1,HE Wei-qing2,WANG Yi-guang2(1.Shenyang Pharmaceutical University1,Shenyang 110015,China;2.Institute of Medicinal Biotechnolgy2,CAMS & PUMC,Beijing 100050,China);Regulatory Mechanism of Morphological Differentiation and Secondary Metabolism in Streptomyces[J];Pharmaceutical Biotechnology;2007-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved