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《Acta Microbiologica Sinica》 2003-01
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Selection of A Strain Producing β-Lactam Antibiotics Acylases*

Zhu Songcheng Huang Xi Zhao Guoping ** Jiang Weihong ** (Laboratory of Molecular Microbiology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China)  
A series of substrates analogues containing the same or similar side chain of its substrate have been synthesized and applied to screen cephalosporin acylase producers from a mass of microorganisms. The deacylation products of these analogues can be detected conveniently and used as screening indicators for the cephalosporin-acylase-producing-microorganism. Six strains possessing deacylation activity have been screened out with these substrate analogs. Among them, strain ZH0650 can simultaneously hydrolyze GL-7ACA, NIPAB and other analogues including AD-NABA. Further investigation on this strain confirmed that it could produce at least three acylases, AD-NABA acylase, penicillin G acylase and cephalosporin acylase, which were characterized by bioassay with multiple substrate analogues. This is the first report that three different acylases were produced by one strain. =
【Fund】: 国家 8 63高技术研究发展计划项目资助 ( 2 0 0 1AA2 35 0 81 )~~
【CateGory Index】: Q939.9
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