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《Journal of Hygiene Research》 2002-04
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Protective effect of extra metallothioneins from rabbit liver induced by zinc on toxicity of lead in rat primary hepatocyte culture

Hao Shoujin,Tian Peiyao,Tang Qingping, Ru Binggen College of Life Science, Peking University, The National Laboratory of Protein Engineering and Plant Genetic Engineering, Beijing 100871,China  
Metallothionein (MT), a low-molecular-weight, cysteine-rich, zinc-binding protein, has an important effect in the detoxification of various metals. However, extra Zn 7-MT protects aginst the cellular toxicity of lead in rat primary hepatocyte (RPHC) culture has seldom been examined thoroughly. This study was therefore designed to determine the effects of extra Zn 7-MT on the toxicity of lead in rat primary hepatocyte culture. Hepatocytes were grown in monolayer culture for 24 h and Zn 7-MT was subsequently added for 24 h. RPHC were exporsed to different concentration of lead for 24 h. Cytotoxity was assessed by enzyme leakage and loss of intracellular K +. Meanwhile, the subcellular distribution and accumulation of lead was studied, and the protactive effect on the cellular toxicity of lead in RPHC culture in the present of Zn 7-MT was investigated. The results showed that the toxicity of lead was significantly less in the presence of Zn 7-MT. The hepatocelluar uptake and accumulation of lead have markedly altered. In the cytosol of control cells, the lead was bound mainly to high-molecular-weight proteins whereas the lead was mainly associated with MT in the presence of extra Zn 7-MT.
【Fund】: 国家九五重点攻关项目 (No .96 -C0 3 - 0 1 - 0 6)
【CateGory Index】: R114
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