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《Journal of Food Science and Biotechnology》 2016-05
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Recovery and Purification of Antroquinonol from Antrodia camphorata Fermentation Products

LU Ruiqiu;ZHANG Bobo;XU Ganrong;School of Biotechnology,Jiangnan University;  
This paper discusses the antroquinonol purification process,the response surface method was used to extract more antroquinonol,and silica gel column chromatography to obtain high purity of antroquinonol.The experimental results show that the optimal conditions for the extraction were as follows:material ratio 1∶25(g/m L),the extraction temperature was 35 ℃,the extraction time was85 min.The best conditions for the first silica gel column chromatography:sample volum 1 ∶40,diameter-length ratio 1 ∶30,elution flow rate 2.0 BV/h,the purity is 70.2%;The second optimum separation was achieved with eluant benzene ∶acetone=7 ∶3(v/v),sample volume at 1 ∶40,diameter-length ratio 1∶10 and elution velocity at 1.0 BV/h,the purity is 91%.
【CateGory Index】: TQ929
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