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Depolymerization of Chitosan and the derivatives by Lipase

Xia Wenshui; Riccardo A A Muzzarelli(Wuxi Unversity of Light Industry)(Faculty of Madicine,University of Ancona)  
Chitosan and derivatives were depolymerized by lipase,with the molecular weight lowering down from ca.700kDa to 13kDa,as shown by the viscosity decrease of its lactate solutions and by the titration of the reducing sugars.Typically,the viscosity was lowered to 35/00 of the initial value after 10 min.Incubation with lipase at 25℃.Measurements taken in the lipase concentration range 4.5×10-3~9.0×10-1 g/L showed a logarithmic dependence of the initial velocity on the enzyme concentration.N-Carboxymethyl chitosan was an even better substrate.
【CateGory Index】: TS201.23
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