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《Journal of Wuyi University(Natural Science Edition)》 2011-01
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Extraction and Determination of(+)-Catechin and Phloridzin from Cynomorium Songaricum

LI Chen1,2,CHEN Wei-lin 3,Guo Mei 3,DI Duo-long 2(1.Instrumental Analysis & Research Center,Wuyi University,Jiangmen 529020,China; 2.Key Laboratory of Chemistry of Northwestern Plant Resources & Key Laboratory for Natural Medicine of Gansu Province,Lanzhou Institute of Chemical Physics,Chinese Academy of Sciences,Lanzhou 730000,China;3.Gansu College of Traditional Chinese Medicine,Lanzhou 730000,China)  
A method for the simultaneous determination of(+)-Catechin and Phloridzin in extracts of Cynomorium songaricum was developed using high performance liquid chromatographic(HPLC) coupled with diode array detector(DAD).A good separation was achieved by using a C18 HPLC column with gradient elution.At the same time,the method for extracting flavonoids from C.songaricum was optimized using the total content of(+)-Catechin and Phloridzin as extraction index and with the ratio of material to liquid,extraction duration and extraction times as influential factors in the orthogonal experiment.The optimum extracting conditions were 7.5 times quantity of methanol to herbal material at 28 kHz and 50 ℃,extraction for three times for 30 min each time.The proposed method was used to extract and determine the two flavonoids in C.songaricum collected from different producing area.The result showed that the method was good in aspects of extraction,precision,accuracy and stability.It can provide a reference to the extraction,analysis and quality control of flavonoids in C.songaricum and its products.
【Fund】: 甘肃省中医药科研课题资助项目(GZK220052015)
【CateGory Index】: TQ464
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