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《Foreign Medical Sciences Section of Pathophysiology and Clinical Medicine》 2005-04
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Lysophosphatidylcholine-induced elevation of asymmetric dimethylarginine level by the NADPH oxidase pathway in endothelial cells

JIA Su-jie, JIANG De-jian, HU Chang-ping, DENG Han-wu, LI Yuan-jian~*(Department of Pharmacology, School of Pharmaceutical Sciences, Central South University, Changsha 410078)  
Objective To explore the mechanism of NADPH oxidase in lysophosphatidylcholine (LPC)-induced elevation of asymmetric dimethylarginine (ADMA). Methods In LPC-treated endothelial cells, the reactive oxygen species (ROS) production, cell viability, the levels of ADMA and NO, the activity of dimethylarginine dimethylaminohydrolase ( DDAH ) and expression of protein arginine methyltransferase I (PRMT I) were detected. Results Treatment with LPC (10μg/mL) for 24h markedly increased the intracellular ROS production, the level of ADMA, the expression of PRMT I, and decreased the concentration of NO and the activity of DDAH. These effects of LPC can be attenuated by diphenyliodonium, the NADPH oxidase inhibitor. Conclusion The increased level of ADMA induced by LPC was due to reduction of DDAH activity and upregulation PRMT expression by stimulation of ROS production via the NADPH oxidase pathway.
【Fund】: 湖南省自然科学基金(02jjy2046)
【CateGory Index】: R54
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