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Development and conformation of a microarray for human gliomas gene expression profiles

SUN Jiyong1,WANG Qi2,YU Hui2,JI Weiyang1,WANG Qing1,LI Bing1,CHEN Kailai1,LI Jiang'an1,LU Xiaojie1 (1.Wuxi Second People's Hospital Affiliated to Nanjing Medical University,Wuxi Jiangsu 214002;2.Shanghai Southern Bio-Medical Tech-Center,Shanghai 200335,China)  
Objective To develop a human glioma-specific cDNA microarray having own intellectual property,and to promote large-scale tumor expression study.Methods According to the previous studies,using 389 gene probes,and optimize various conditions of gene chips: slice-base,sample liquid,sample size,cross-linking and signal light strength.At last 20 tumor samples were tested to validate the products.Results A useful and dependable microarray product was developed and proved to be a high throughput method of gene expression profiles assay.Conclusion Microarray product is a kind of specificity,sensitivity,quantitative tools for gene expression profiles study,and may be a great methods in gliomas biology research.
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