Identii cation of inl ammation factors related to radioresistance of nasopharyngeal carcinoma by an antibody array
LI Jiaoyang;QU Jiaquan;YI Hong;YI Hongmei;XIAO Zhiqiang;Key Laboratory of Cancer Proteomics of Ministry of Health of China, Xiangya Hospital, Central South University;
Objective: To screen inflammation factors related to radioresistance of nasopharyngeal carcinoma(NPC). Methods: Antibody array of human inflammation factors(AAH INF 3) were used to compare the expression dif erences between radioresistant NPC CNE2 IR and radiosensitive NPC CNE2 cells. Immunohistochemistry was performed to detect the expression levels of differential inflammation factor IL 8 in the radioresistant and radiosensitive NPC tissues(30 cases for each group). Results: The expression levels of IL 8, granulocytemacrophage colony stimulating factor(GM CSF) and intercellular adhesion molecule 1(ICAM 1) in CNE2 IR cells were significantly higher than those in CNE2 cells, and CNE2 IR cells secreted more IL 8 and GMCSF than CNE2 cells while secreted lesser matrixmetalloproteinase 2(TIMP 2) than that in CNE2 cells. Immunohistochemistry showed that the expression levels of IL 8 were signii cantly higher in the radioresistant NPC tissues than those in the radiosensitive NPC tissues. Conclusion: Abnormal expression or secretion of IL 8, GM CSF, ICAM 1, and TIMP 2 may play an important role in NPC radioresistance, which may provide a novel clue for further investigating the molecular mechanism of NPC radioresistance.