Role of p38MAPK in asymmetric dimethylarginine induced cytoskeleton changes in endothelial cells
ZHANG Changqin;ZHANG Dongliang;LIU Wenhu;Department of Nephrology, Beijing Tiantan Hospital ai liated to Capital Medical University;Department of Nephrology, Beijing Friendship Hospital ai liated to Capital Medical University;
Objective: To explore the effect of asymmetric dimethylarginine(ADMA) on the cytoskeleton of human umbilical vein endothelial cells(HUVECs) and the role of p38 mitogen activated protein kinase(p38MAPK) in this process. Methods: HUVECs were cultured in vitro and divided into four groups: a control group, a SB203580 group, a ADMA group( including a subgroup of dose ef ect relationship and a subgroup of time ef ect relationship), and a SB203580+ADMA group(including a subgroup of dose ef ect relationship and a subgroup of time ef ect relationship). h e treated cells were stained by immunol uorescence, then the conformational changes of F actin of cells in all groups were observed under confocal microscope. h e grey scale values of acquired imageswere analyzed by image analysis software and the fluorescence of F actin was quantified by flow cytometry. Results: ADMA can increase the formation of stress i bers, then resulted in the increase of the grey scale values and l uorescent quantii cation of F actin. SB203580 can inhibit the ef ect of ADMA on cytoskeleton. Conclusion: ADMA can induce the cytoskeleton changes in endothelial cells in a concentration or time dependent manner, which is involved in p38MAPK pathway.
【CateGory Index】： R329.2