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《Journal of Clinical and Pathological Research》 2015-03
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The expression of GAP-43 after intraocular transplantation of olfactory ensheathing cells(OECs) transfected with ciliary neurotrophic factor(CNTF) mediated by adenovirus after optic nerve injury in rats

LIU Baoguo;ZHANG Long;HUANG Wei;XU Hong;HE Liming;XIAO Gang;Department of Neurosurgery,Yuebei People's Hospital;Department of Pathology, Yuebei People's Hospital;  
Objective: To investigate the promoter action of intraocular transplantation of olfactory ensheathing cells(OECs) transfected with ciliary neurotrophic factor(CNTF) mediated by adenovirus intraocular on Long distance regeneration of nerve fibers of rats after optic nerve injury by detecting the express of GAP-43. Methods: Intracranial optic nerve injury model of rats were established by the microsurgery through subfrontal approach and the rats were averagely divided into different groups. OECs transfected with CNTF mediated by adenovirus was transplanted into the rats' vitreum and the integral optical density value of GAP-43 expression was detected. Results: OECs steadily secreting CNTF mediated by adenovirus was successfully isolated and cultured. The expression of GAP-43 in CNTF transplantation group and OECs-CNTF transplantation group was significantly higher than the control groups(P0.01), and the expression of GAP-43 in CNTF transplantation group was much higher than OECs-CNTF transplantation group(P0.05). Conclusion: There is obvious promoter action of intraocular transplantation of OECs transfected with CNTF mediated by adenovirus intraocular on Long distance regeneration of nerve fibers of rats at er optic nerve injury.
【Fund】: 广东省自然科学基金(10151202602000001);; 广东省医学科研基金(A2012680);; 韶关市医药卫生科研计划项目(Y12145)~~
【CateGory Index】: R774.6
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【Co-citations】
Chinese Journal Full-text Database 5 Hits
1 LI Jing;ZHU Lei;LIN Hao-dong;CHEN Ai-min;Department of Trauma and Reconstruction Surgery,Changzheng Hospital,Second Military Medical University;;Protective effect of exogenous cardiotrophin-1(CT-1)against spinal cord injury in rats[J];Academic Journal of Second Military Medical University;2014-07
2 Xia-Ru Zhu;Xiao-Ming Chen;Eye Center,West China Hospital of Sichuan University;;Recent research progress of traumatic optic nerve injury in rat model[J];International Eye Science;2014-12
3 Guangyao Liu;Rui Jiang;Yan Jin;Department of Orthopedics,China-Japan Union Hospital,Jilin University;Department of Ophthalmology,Second Hospital of Jilin University;;Sciatic nerve injury repair: a visualized analysis of research fronts and development trends[J];中国神经再生研究(英文版);2014-18
4 WANG Jianping;WANG Xinxin;JIANG Chao;Department of Neurology,The Fifth Affiliated Hospital of Zhengzhou University;;Study on the mechanisms of transplanted bone marrow mononuclear cells for white matter repair in cerebral in- farction mice[J];Journal of Apoplexy and Nervous Diseases;2013-11
5 Li Bing-nan;Li Wei-dong;Lin Jun-tang;Feng Hui-gen;Department of Life Sciences and Technology, Xinxiang Medical University;;Construction and identification of bicistronic eukaryotic expression vector of human brain-derived neurotrophic factor and neurotrophine-3[J];Chinese Journal of Tissue Engineering Research;2014-15
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