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《Journal of Clinical and Pathological Research》 2015-03
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The investigation of the relationship between HSP70 and TLR-NF-κB during the process of acute lung injury

CAO Zheng;ZHU Jian;LU Ping;SHENG Jing;Division of Geriatrics, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine;  
Objective: To investigate the possible relationship between HSP70 and Toll-NF-κB during the process of acute lung injury induced by LPS. Methods: Totally 32 adult male Wistar rats were randomly divided into two groups as follows: saline control group(NS group, 8) and lipopolysaccharide group(LPS group, 32). Then divide LPS group into three subgroup by different time length(2/6/24 h), each group has 8 rats. ALI was induced by injecting LPS intravenously(6 mg/kg) in the LPS group. Arterial blood gas analysis, lung tissue wet/dry weight ratio, lung histopathological changes under light microscope and the scores of histological injury in each group were observed. Serum was collected to detect the released level of TNF-α, IL-6, IL-10 by enzymelinked immunosorbent assay(ELISA). Real time-PCR was used to analyse the expression of HSP70, TLR and NF-κB in lung tissue. Results: No significant difference was shown in the results of NS group. As time goes on, the concentration of HSP 70 in pulmonary in LPS group was positively correlated with the concentration of TLR and NF-κB(P0.05); the results of blood gas analysis suggests an progressive hypoxia and carbon dioxide retention; the W/D ratio in pulmonary raise gradually. Conclusion: During the process of rat's acute lung injury, there's a one-to-one relationship between HSP70 and Toll-NF-κB.
【Fund】: 上海市第九人民医院院级课题(JY2011A06)~~
【CateGory Index】: R563
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【References】
Chinese Journal Full-text Database 1 Hits
1 LIU Zi-Chen;WU Di;WANG Gang;Xingtai Medical College;The Third Hospital of Xingtai;;Effect of Ginaton injection on the expression of NF-κB in rats with acute lung injury induced by LPS[J];安徽医药;2015-12
【Citations】
Chinese Journal Full-text Database 2 Hits
1 WANG Qiao,ZHU Hong,ZHOU Longnü,QIAN Li. Department of Emergent Medicine,Shanghai Ninth People's Hospital,Shanghai Jiaotong University School of Medicine,Shanghai 200011,China;Expression of heat shock protein 70 and drug intervention in rats with septic acute lung injury[J];上海医学;2010-10
2 LI Jin-shi reviewer BAI Jian-wen reviser (Department of Emergency,Oriental Hospital,Tongji University,Shanghai 200120,China);Research advances in Toll-like receptors and aute lung injury[J];上海交通大学学报(医学版);2007-07
【Co-citations】
Chinese Journal Full-text Database 8 Hits
1 LIU Zi-Chen;WU Di;WANG Gang;Xingtai Medical College;The Third Hospital of Xingtai;;Effect of Ginaton injection on the expression of NF-κB in rats with acute lung injury induced by LPS[J];安徽医药;2015-12
2 XU Tong;WANG Cun-lian;XU Ming-ju;ZHANG Rui-hua;LIU Bao-jian;WEI Dong;WANG Guo-hua;Key Laboratory of Preventive Veterinary Medicine,Department of Veterinary Medicine,Animal Science College,Hebei North University;;Effect of Epigallocatechin Gallate on Toll-like Receptor-4 Expression in Mouse Model of Acute Lung Injury Induced by H9N2 Swine Influenza Virus[J];畜牧兽医学报;2015-08
3 CAO Zheng;ZHU Jian;LU Ping;SHENG Jing;Division of Geriatrics, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine;;The investigation of the relationship between HSP70 and TLR-NF-κB during the process of acute lung injury[J];临床与病理杂志;2015-03
4 WANG Xue-qin1,YUE Zhong-yong2,LI Ke-zhong1,MA Jin-feng1(1.Department of Anesthesiology,The Second Hospital of Shandong University,Jinan 250033,China;2.Department of Anesthesiology,Pingdu People’s Hospital,Pingdu 266001,Shandong,China);Effects of cyclic mechanical stretch on the expression of TLR4 in alveolar macrophages of rats[J];山东大学学报(医学版);2013-06
5 SHEN Xiao-hong1 HE Miao1 XIONG Xu-dong1 PENG Wen-bo2 ZHANG Yan-ting1 1.Shanghai Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine; 2.The 6th People's Hospital Affiliated to Shanghai Jiaotong University;Regulatory Effect of "Yantiao Decoction" on Lung HSP70 and NF-κB in Sepsis Rats[J];上海中医药大学学报;2013-03
6 QIAN Zhi-bin1,WANG Yu-xia2,LIU You-cai1,JI Ming-li2(1.Functional Laboratory;2.Department of Pathophysiology,Xinxiang Medical University,Xinxiang,Henan 453003,P.R.China);The relationship between expressions of IFN-γ and change of Phrenic nerve discharge curve in rabbits with oleic acid induced acute lung injury[J];中国现代医学杂志;2012-05
7 YANG Ai-dong,LI Wen-wen,WANG Li-xia,WANG Dong-ying,WU Zhong-hua,SU Zhong-hao,Pang Hui-fang(Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China);Expression of TLR4 and CD14 mRNA in rats with acute lung injury induced by lipopolysaccharide[J];中国实验动物学报;2011-03
8 WANG Qiao,ZHU Hong,ZHU Jian,ZHOU Long-nv Department of Emergency Medicine,the Ninth People's Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200011,China;Dynamic expression of Toll-like receptor 4 in septic acute lung injury in rats[J];中华实用诊断与治疗杂志;2011-02
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