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《Journal of Clinical and Pathological Research》 2015-07
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Detecting the EGFR mutation status of malignant pleural effusion by immunohistochemistry

GUO Haihua;GUO Ting;HUANG Lijun;ZHANG Zhipei;WANG Lei;The Jvyuan Center Hospital of Chenggu Country;Department of Thoracic Surgery, Tangdu Hospital, the Fourth Military Medical University;  
Objective: To assess the availability of immunohistochemistry(IHC) for detection of EGFR mutation status of malignant pleural effusion. Methods: From January 2012 to December 2013, 154 patients with lung adenocarcinoma were enrolled into this research, and amplification block mutation system technology(ARMS) and IHC were used to detect the mutation status of malignant pleural effusion. The sensitivity, specificity and concordance rate were compared between the ARMS and IHC methods. Results: A total of 85 patients with average age of 66.4 years were selected into this study finally. The overall mutation rate tested by ARMS method was about 49.4%, including 17.6% of E746_A750 del and 22.4% of L858 R. As compared with ARMS, the IHC method contributed the sensitivity of E746_A750 del and L858 R with 93.3% and 89.5%, respectively. Furthermore, IHC devoted an overall mutation rate of 41.2% in detecting exfoliative cells of malignant pleural effusion, involving the sensitivity of 83.3% for E746_A750 del and 66.7% for L858 R, which had a specificity of 100%. Conclusion: IHC was a reliable and precise tool for detecting the EGFR mutation status in malignant pleural effusion, which can be used as a screening method.
【Fund】: 第四军医大学唐都医院基础研究创新基金和苗子人才基金~~
【CateGory Index】: R734.2
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【References】
Chinese Journal Full-text Database 1 Hits
1 GUO Ting;WANG Lei;LI Xiaofei;Department of Thoracic Surgery,Tangdu Hospital Affiliated Fourth Military Medical University;;Research progress in the detection technology of EGFR mutations in non-small cell lung cancer[J];临床与病理杂志;2017-09
【Citations】
Chinese Journal Full-text Database 2 Hits
1 Chang LIU;Dongbo XU;Diansheng ZHONG;Department of Medical Oncology, Tianjin Medical University General Hospital;Department of Pathology,Tianjin Medical University;;Immunohistochemical Detections of EGFR Mutations in NSCLC[J];中国肺癌杂志;2014-09
2 Qing MA;Jing WANG;Diansheng ZHONG;Chao NING;Chang LIU;Ping XIAO;Department of Medical Oncology, Tianjin Medical University General Hospital;Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital;;Value of Immunohistochemical Staining with Mutation-specific Antibodies in Detecting EGFR Mutations: A Meta-analysis[J];中国肺癌杂志;2014-06
【Co-citations】
Chinese Journal Full-text Database 4 Hits
1 WANG Qiang;FAN Xiaoyun;XU Ke;WANG Rui;NING Yajing;CAO Liyu;Department of Geriatric Respiratory Medicine,The First Affiliated Hospital of Anhui Medical University;Department of Pathology,The First Affiliated Hospital of Anhui Medical University;;Association of EGFR-19,EGFR-21 and TTF-1 expression with the Clinical features in patients with lung adenocarcinoma[J];安徽医药;2017-07
2 YANG Dongyan;TIAN Ying;Shaanxi Provincial Hospital of Traditional Chinese Medicine;Tianjin University of Traditional Chinese Medicine;;HuaJi Tang Combined with Goserelin in the Treatment for 53 Cases of Hysteromyoma[J];西部中医药;2016-12
3 GUO Haihua;GUO Ting;HUANG Lijun;ZHANG Zhipei;WANG Lei;The Jvyuan Center Hospital of Chenggu Country;Department of Thoracic Surgery, Tangdu Hospital, the Fourth Military Medical University;;Detecting the EGFR mutation status of malignant pleural effusion by immunohistochemistry[J];临床与病理杂志;2015-07
4 ZHANG Bo;WANG Bing;WANG Yongqiang;Tianjin University of Traditional Chinese Medicine;Tianjin First Center Hospital;Tianjin Huanhu Hospital;;Study of Rhubarb Tannins Effect on Acute Encephaledema by Rats with Encephaledema Followed by Traumatic Brain Injury[J];中国中医急症;2015-03
【Co-references】
Chinese Journal Full-text Database 1 Hits
1 GUO Haihua;GUO Ting;HUANG Lijun;ZHANG Zhipei;WANG Lei;The Jvyuan Center Hospital of Chenggu Country;Department of Thoracic Surgery, Tangdu Hospital, the Fourth Military Medical University;;Detecting the EGFR mutation status of malignant pleural effusion by immunohistochemistry[J];临床与病理杂志;2015-07
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 YANG Jingping △,ZHANG Yan, ZHONG Yanfeng, ZHENG Jie (Department of Pathology,Peking University School of Basic Medical Sciences,Beijing 100083,China);Effects of different pH values of fixatives and antigen retrieval buffers on immunohistochemical staining[J];北京大学学报(医学版);2003-01
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