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《Journal of Wuhan Botanical Research》 2006-01
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Cytological Identification on the Interspecific Hybrid of Zea mays and Zea diploperennis

LI Cong-Qiang~1,LIN Gang~1,LI Ke-Xiu~1,SONG Yun-Chun~2,XIONG Zhi-Yong~1,HE Guang-Yuan~1~~*(1.China-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory,Huazhong University of Science and Technology,Wuhan 430074,China;2.Key Laboratory of MOE for Plant Developmental Biology,Wuhan University,Wuhan 430072,China)  
Fluorescence in situ hybridization(FISH) was carried out on mitotic chromosomes of Zea diploperennis(DP),maize inbred lines F107,GB57 and their hybrids using knob-associated tandem repeats,180-bp repeats and TR-1 elements,together with 45S rDNA as probes.The distribution patterns of the three repeated sequences were analyzed on parental chromosomes.The hybrids of maize and DP were(identified by) FISH.According to the hybridization signals on chromosome positions,the origin of foreign idioplasm was confirmed.The application using knob-associated tandem repeats as excellent chromosomal markers on detecting the integration of foreign chromosome and the chromosomal activity at meiosis was also discussed.
【Fund】: 国家973计划项目(2002CB111302)资助;; 国家自然科学基金资助项目(30370807);; 湖北省自然科学基金重点项目资助(2002AB088)
【CateGory Index】: S513
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