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《Journal of Wuhan Botanical Research》 2006-01
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Analysis of Total Proteins in Pollen of Humulus scandens Lour. in Wuhan Region of China by Two-dimensional Electrophoresis

LI Dong-Dong~(1,2*),HE Shao-Heng~2,TAO Ai-Lin~2(1.Department of Bioengineering,Hainan University,Haikou,Hainan 570228,China;2.Institute of Allergy and Inflammation,Medical College of Shantou University,Shantou,Guangdong 515031,China)  
The total proteins in pollen of Humulus scandens Lour.,one of the most popular aeroallergen in China,were analyzed by two-dimensional electrophoresis in current study.The total proteins of the pollen were extracted by trichloracetic acid(TCA) method,and then separated by isoelectric focusing as first dimension and SDS-PAGE as the second dimension.The spots of proteins were visualized by staining with Coomassie Brilliant Blue.After analysis with software(ImageMaster 2D),122 different proteins were detected;their isoelectric point(pI),Molecular weight(MW) and relative volume of each protein in the pollen were also discovered.This is the first high-resolution,two-dimensional protein map of the pollen of H.scandens Lour.in China.Our finding has built a solid foundation for identification,characterization,gene cloning and standardization of allergenic proteins in the pollen of H.scandens Lour.in further.
【Fund】: 国家自然科学基金资助项目(30560092);; 海南省自然科学基金资助项目(80406);; 海南省教育厅高校科研资助项目(Hjkj200510)
【CateGory Index】: S567.219
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