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《Journal of Wuhan Botanical Research》 2008-02
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The Inhibitory Effects in vitro of Ethanol Extracts from the Fruit of Akebia trifoliata on the Tyrosinase Activity

PENG Di-Fei1,ZHONG Cai-Hong1,2,ZHOU Hai-Yan3,ZEN Bin1,WANG Zhong-Yan1 (1.Hunan Horticultural Research Institute,Changsha 410125,China;2.Wuhan Botanical Garden,The Chinese Academy of Sciences,Wuhan 430074,China;3.Biochemistry and Fermenting Engineering Laboratory,Hunan Agricultural University,Changsha 410128,China)  
Fruits of cultivated Akebia trifoliata were used as experimental materials and the active components were extracted with 80% ethanol from pericarp,flesh and seeds respectively.The ethanol extracts were obtained by centrifuging and filtering,distilling and concentrating on revolving evaporation apparatus at reduced pressure,and then vacuum drying.The inhibitory activities of these extracts on tyrosinase were examined with LDOPA as substrate and arbutin as positive control.The results showed that the extracts from different parts of akebia fruit all had obvious inhibitory activity on tyrosinase,the strongest effects occurred with flesh extract.Flesh extract at 200 μg/mL had similar level of effects to arbutin at 270 μg/mL concentration.
【Fund】: 湖南省自然科学基金资助项目(05JJ30030);; 湖南省农业科学院重点学科建设项目资助
【CateGory Index】: R285
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