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《Journal of Wuhan Botanical Research》 2008-02
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Peroxidase Isozyme of Young Leaves of Genus Fagopyrum Plants at Three-leaf Stage

ZHANG Yi-Zhong1,2,CHEN Qing-Fu1* (1.Institute of Plant Genetics and Breeding,School of Biological Technology and Engineering,Guizhou Normal University,Guiyang 550001, China;2.Department of Environment and Life Science,Bijie College,Bijie,Guizhou 551700,China)  
The peroxidase isozyme of twenty-eight accessions of cultivated and wild buckwheat belonging to eight species of genus Fagopyrum including seven species of the big-achene group and one species of the small-achene group were studied by means of po-lyacrylamide gel eletrophoresis(PAGE).The results showed that there are a total of twenty-three bands of peroxidase isozyme,and the variation of band number from four to nine among different buckwheat species.F.esculentum and F.tataricum have a total of six bands and nine bands,respectively.The results about the zymograph and the clustering showed that there are much great difference of zymographs between the big-achene group and the small-achene group and that F.megaspartanium and F.pilus are close to F.esculentum and F.tataricum,respectively,supporting Chen's hypothesis that F.megaspartanium and F.pilus may be the ancestor species of F.esculentum and F.tataricum,respectively.
【Fund】: 国家自然科学基金资助项目(30270852 30471116);; 教育部新世纪人才支持计划项目(NECT2004-0931);; 国家“十一.五”科技支撑计划项目(2006BAD02B06)
【CateGory Index】: Q946
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