Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Wuhan Botanical Research》 2008-05
Add to Favorite Get Latest Update

Application of Poloxamer in Fat-soluble Allelochemicals Bioassay

TAO Yue-Hong, ZHANG Zhao, ZHANG Ben-Gang, SI Jian-Yong(Institute of Medicinal Plant Development, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100094, China)  
During bioassay for the fat-soluble allelochemicals,we generally use common organic solvents such as ethanol etc.to solubilize the fat-soluble substances,for the method,it easily causes toxic effects on seeds and influence accuracy of the bioassay.On the principle of pharmacy,poloxamer (F-68) is adopted in our research,which is a kind of surfactant used in animal and cytological experiments,as a solubilizer to the experiment of fat-soluble allelochemicals bioassay.We compared the activity of vanillin and ferulic acid with two methods of the poloxamer and ethanol solution in general.The result showed that the concentration of allelochemicals solution processed by poloxamer had no change in quantitative detection of allelopathy activity,and no harmful effect on seed germination and seedling growth,and making for quantitative studies to the activity of allelochemicals.In contrast with ethanol groups,we found that the activity of allelochemicals has been strengthened,or weakened which caused by over concentration from ethanol volatile,to the extent that the allelochmicals crystallized from the solution.The research indicated that the method which the allelochemicals processed by poloxamer has adventages than the general method in fat-soluble allelochemicals.
【Fund】: 国家中医药管理局科学技术研究专项(04-05ZP07)“濒危中药材关黄柏野生资源抚育基础研究”;; 北京市教委重点学科共建项目(XK100230448)“濒危药用植物黄檗野生种群自我更新障碍机制研究”
【CateGory Index】: Q946
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 1 Hits
1 DAI Ling-Chao,TAO Yue-Hong,ZHANG Zhao*,ZHANG Ben-Gang,SI Jian-Yong,LIU Yan-Lu(Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union College,Beijing 100193,China);Research on Surfactants Application in Safety of Two Crops[J];Plant Science Journal;2011-03
Chinese Journal Full-text Database 10 Hits
1 LIN Jie1, ZENG Rensen2, SHI Mubiao1, CHEN Zhan1, LIANG Ziheng1(1 College of Food Science, South China Agric. Univ., Guangzhou 510642, China;2 Institute of Tropical and Subtropical Ecology, South China Agric. Univ., Guangzhou 510642, China);Allelopathic Effects of Eucalyptus urophylla and Pinus elliottii on Pisolithus tinctorius[J];Journal of South China Agricultural University;2003-02
2 LIANG Bin1,YUAN Ya-li1*,GU Wen-xiang2,LI Yong-jun2(1.The Department Of Life Sciences,NanHua University,Hengyang Hunan 421001 2.College Of Sciences,South China Agric Univ,Guangzhou 510642,China);Studies on the Allelopathic Effects of Mikania micrantha[J];Jiangxi Plant Protection;2006-01
3 Wang Pu Zhao Xiuqin(College of Crop Science, CAU);Effect of Allelochemicals on Cotton Seed Germination and Seedling Growth[J];Journal of China Agricultural University;2001-03
4 MA Rui Xia,FENG Yi,LI Xuan (Research Center for Eco\|Environmental Sciences,Chinese Academy of Sciences,Beijing,100085\ China);Effects of allelochemicals on growth of Bacillus subtilis and its denitrification under anaerobic condition[J];ACTA ECOLOGICA SINICA;2000-03
5 LI Xinyu 1, PIAO Shunji 2, TANG Haiping 1, WU Meihua 2 ( 1Institute of Resources Science, Beijing Normal University, Beijing 100875, China; 2Department of Ecology and Environmental Science Inner Mongolia University, Huhhot 010021, China).;Effect of alkaloid from Artemisia sieversiana flower on the apparatus division of rhizome knot of Leymus chinensis[J];Chinese Journal of Ecology;2004-02
6 YANG Qihe~1,YE Wanhui~2,LIAO Fulin~1,YIN Xiaojuan~1 (~1Jiaying University,Meizhou 514015,China;~2South China Botanical Garden,Chinese Academy of Sciences,Guangzhou 510650,China).;Effects of allelochemicals on seed germination.[J];Chinese Journal of Ecology;2005-12
7 ZHAO Cai-xia, ZHAI Mei-zhi,WANG Wei and BIE Zhi-xin (Forestry College, Northwest A & F University, Yangling Shaanxi 712100,China; Yangling Vocational and Technical College, Yangling Shaanxi 712100, China);The Allelopathy of Walnut Green Husk I :Effects of the Secondary Substances on the Growth of the Seedlings[J];Acta Agriculturae Boreali-occidentalis Sinica;2005-06
8 HE Jun~1, WANG San-gen~1, DING Wei~2 (1. College of Agronomy and Life Science, Southwest Agricultural University, Chongqing 400716, China; 2. College of Plant Protection, Southwest Agricultural University, Chongqing 400716, China);STUDY ON ALLELOPATHIC EFFECTS OF ALCOHOL EXTRACTS FROM ARTEMISIA ANNUA L.[J];Journal of Southwest Agricultural University;2004-03
9 Ma Yueqiang, Liao Liping, Yang Yuejun, Wang Silong, Gao Hong, Chen Chuying (Institute of Applied Ecology, Academia Sinica, Shenyang 110015).;Effect of vanillin on the growth of Chinese fir (Cunninghamia lanceolata) seedlings.[J];CHINESE JOURNAL OF APPLIED ECOLOGY;1998-02
10 MEI Lingxiao,CHEN Xin,TANG Jianjun(College of Life Sciences,Zhejiang University,Hangzhou 310029,China).;Allelopathic effects of invasive weed Solidago canadensis on native plants.[J];Chinese Journal of Applied Ecology;2005-12
Chinese Journal Full-text Database 10 Hits
1 Dong Yuan1 Chen Wei1 Zhao Yongxu1 Jiang Yonghou1 Shang Hanwu2,1* (1Institute of Bioengineering,Zhejiang Sci-Tech University,Hangzhou 310018,China,2College of life sciences,China Jiliang University,Hangzhou,Zhejiang 310018);Allelopathy of several exotic invasive weeds and prospect of its application[J];Anhui Agricultural Science Bulletin;2007-05
2 Hu Zongqing(Fujian Forestry Professional and Technological College,Nanping 353000);The Effect of Allelochemicals of Pinus massoniana root on its seed germination[J];Anhui Agricultural Science Bulletin;2007-18
3 Liu Bao et al.(1 Fujian Agriculture and Forestry University,Fujain Fuzhou 350002,China;2 Fujian Chinese fir research institute,Fujain Fuzhou 350002,China);Allelopathic Effect of Allelochemicals of Chinese fir Stump on Germination of Chinese fir seed after decomposed six monthes[J];Anhui Agricultural Science Bulletin;2008-21
4 ZHANG Jun-ying et al (College of Resources and Environmental Sciences,Hebei Polytechnic University,Tangshan,Hebei 063009);Development of Plant Allelopathy[J];Journal of Anhui Agricultural Sciences;2007-21
5 ZHANG Bo et al (College of Life Science, Northwest Normal University, Lanzhou, Gansu 730070 );Preliminary Study on the Allelopathy of Important Chinese Medicinal Herbs in Gansu Province[J];Journal of Anhui Agricultural Sciences;2008-02
6 LI Jing et al(College of Life Science,Northwest Normal University,Lanzhou,Gansu 730070);Research on the Autointoxication of Angelica sinensis[J];Journal of Anhui Agricultural Sciences;2008-03
7 XIE Yuan et al(Department of Gardens,Sichuan Agricultural University,Ya′an,Sichuan 625014);Effect of Trifolium repeus Infusion on the Seed Germination and Seedling Growth of Festuca arundinacea[J];Journal of Anhui Agricultural Sciences;2008-09
8 WANG Yu-lin et al (Chemistry Department,Qiannan Normal College for Nationalities,Duyun City,Guizhou 558000);The Biological Characteristics of Alien Plant Anredera cordifolia and Its Control[J];Journal of Anhui Agricultural Sciences;2008-13
9 WANG Hong-wei et al(College of Plant Protection,Henan Agricultural University,Zhengzhou,Henan 450002);Effects of Extracted Liquid from Datura stramonium on Seed Germination and Seedling Growth of Vegetables[J];Journal of Anhui Agricultural Sciences;2008-35
10 HE Cheng-zhong et al(Key Laboratory of Biodiversity Conservation in Southwest China,State Forestry Administration,Southwest Forestry University,Kunming,Yunnan 650224);Effect of the Water Extracts from Physic Nut Leaves on Seed Germination[J];Journal of Anhui Agricultural Sciences;2009-10
China Proceedings of conference Full-text Database 8 Hits
1 LU Ming 1,2,WANG Lin-bo 1,HAN-Peng 3,CHEN Zhu-kang 1,YANG Mei-cheng 1,CHEN Gui-liang 1 (1.Shanghai Institute for Food and Drug Control,Shanghai,201203,China;2.Second Military Medical University,Shanghai 200433,China 3.Chinese Pharmacopoeia Commission,Beijing 100061,China);Research of the method to measure the dynamic viscosity of Polyacrylic Resin Ⅱ[A];[C];2011
2 WANG Zhaohong (Guangyuan Food and Drug inspection office,Guangyuan 628000,Sichuan,China);Analysis of Hydrochloric Acid Berbamine Sugarcoating Piecespilt and Countermeasures to the Spilt[A];[C];2009
3 Wang Quanjie1,2,Ren Fangping 1,Gao Long 2 (1.School of chemical and material,Dalian Polytechnic University,Dalian 116000,China 2.Chemistry and Biology College,Yaitai University,Yantai 264005,China;);Research Progress of Comprehensive Utilization of Walnut Seedcases[A];[C];2010
4 TIAN Lei~1,YANG Da-kai~2,CHEN Wu~2 1 Jiangsu Provincial Hospital of Traditional Chinese Medicine,Nanjing 210029,China 2 Nanjing University of Traditional Chinese Medicine,Nanjing 210029,China;Study of Permeation Enhancers of shangkexiaoyan Cataplasm[A];[C];2010
5 Jian Xiaona~1 HU Ming~2 Jiang Xuehua~(*2) (1.Chengdu Pharmaceutical Co.,Diao Group;2.West China School of Pharmacy,Sichuan University);The study of management about approed oral solid chemical drug's formulation and manufacture process improvement[A];[C];2009
6 HAI Tang,JIA Xian-yan,ZHANG Xiao-feng (College of Ecology and Environment,Inner Mongolina Agriculture University,Huhhot,010019 China);Alfalfa varieties of receptor extract the impact of grass seed germination[A];[C];2009
7 Zhaohong WANG (Guangyuan Food and Drug inspection office,Guangyuan,628000,China);Analysis of hydrochloric acid berbamine sugarcoating piece spilt and countermeasures to the spilt[A];[C];2009
8 Zhang Miru Li Xiangju Li Yongjun(Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100094,China);Effect of aqueous extract of Flaveria bidentis on plant germination[A];[C];2007
Chinese Journal Full-text Database 10 Hits
1 HUI Ji-rui et al (College of Life Science, Northwest Normal University, Lanzhou, Gansu 730070);Study on Autotoxicity of Angelica sinensis (Oliv.) Diels in Different Development Stages[J];Journal of Anhui Agricultural Sciences;2008-02
2 LI Jing et al(College of Life Science,Northwest Normal University,Lanzhou,Gansu 730070);Research on the Autointoxication of Angelica sinensis[J];Journal of Anhui Agricultural Sciences;2008-03
3 LI Xiao-ling et al (Biological Medicine Engineering Department of Shangluo University,Shangluo GAP Research Engineering Center for Traditional Chinese Medicine,China Academy of Traditional Chinese Medicine,Shangluo,Shaanxi 726000);Research Progress on Allelopathy of Medicinal Plants[J];Journal of Anhui Agricultural Sciences;2009-05
4 YIN Qi-lin et al(College of Urban Construction and Environment,Anhui Science and Technology University,Fengyang,Anhui 233100);Research Progress on the Continuous Cropping Obstacles of Plants Caused by Phenolic Acids[J];Journal of Anhui Agricultural Sciences;2011-34
5 LIU Bao-dong1,WANG Yue2(1.College of Forestry Beihua University,Jilin,Jilin 132013;2.Yijiadian Forestry Workstation of Songyuan,Songyuan,Jilin 131200);Chemical Properties Change of Soil after Ginseng Cultivation and the Effect of its Extracts on Germination of Crop Seeds[J];Northern Horticulture;2010-22
6 Fu Jia, Wang Yang, Yan Xiufeng(Northeast Forestry University, Harbin 1500 40, P. R. China);The Eco-physiological Function and Economic Value of Terpenoids[J];Journal of Northeast Forestry University;2003-06
7 Yang Jingchun, Li Zhiping and Shakayi-Ayakou;ROOT SECRETIONS OF GINSEN AND THEIR EFFECTS ON THE MICROBES IN RHIZOSPHERE OF GINSENG[J];Journal of Northeast Normal University;1982-01
8 CHAI Qiang,HUANG Gao-bao (Agroecology Engineering Institute of Gansu Agricultural University,Lanzhou,730070,China);Review on action mechanism affecting factors and applied potential of allelopathy[J];Acta Botanica Boreali-occidentalia Sinica;2003-03
9 ZHANG Chen-lu,SUN Qun~*,YE Qing(College of Life Science,Northwest Sci-Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China);Obstacle Effect of Continuous Cropping on Salvia miltiorrhiza Growth[J];Acta Botanica Boreali-occidentalia Sinica;2005-05
10 CAI Zu-guo~1,XU Xiao-biao~2,ZHAO Yi-peng~1,ZHOU Hui-ping~2(1 Department of Horticulture,Henan College of Science and Technology,Xinxiang,He'nan 453003,China;2 College of Agronomy,Jiangxi Agricultural University,Nanchang 330045,China);Ultrastructure of in vitro Stem Apex of Kivi in Cryopreservation[J];Acta Botanica Boreali-Occidentalia Sinica;2006-08
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 LIU Yan,LIN Si zu,CAO Guang qiu, DU Ling( Fujian Agriculture and Forestry University,Nanping 353001,China);Isolation and Bioassay of Active Allelochemicals from Chinese fir and it's Associated Tree Species[J];Journal of Fujian College of Forestry;2001-03
2 You Huaming Lin Sinzu Huang Zhiqin Huang Siguo Cao Guangqiu (Fujian College of Forestry, Nanping 353001);Effects of the Aquatic Extractor of Some Common Plant Species on Chloroplast of Chinese Fir[J];JOURNAL OF FUJIAN COLLEGE OF FORESTRY;1998-04
3 Huang Zhiqun (Institute of Applied Ecology, Academia Sinica, Shenyang 110015) Lin Sizu , Cao Guangqiu (Center of Chinese fir Research, Fujian College of Forestry, Nanping 353001);Effect of the Aqueous Extracts of Phyllostachys edulis and Castanopsis schlasophylla on the Germination of Chinese fir Seeds[J];JOURNAL OF FUJIAN COLLEGE OF FORESTRY;1999-03
4 Zeng Rensen LuoShiming (Agroeoology Lab, South China Agr. Univ. );STUDY ON ALLELOPATHIC POTENTIALS OF Cymbopogon citratus, Ageratum conyzoides AND Bidens pilosa[J];Journal of South China Agricultural University;1993-04
5 HE Yan biao 1, ZHANG Mao xin 1, HE Ting yu 2, GU Wen xiang 2 (1 College of Resources and Environment, South China Agric. Univ., Guangzhou 510642, China; 2 College of Sciences, South China Agric. Univ., Guangzhou 510642, China);Studies on the Allelopathic Effects of Chromolaena odoratum[J];Journal of South China Agricultural University;2002-03
7 Cao Panrong;Lou Shiming(Dept.of Agronomy,South China Agr.Univ,Guangzhou,610642);STUDIES ON THE ALLELOPATHY OF Eucalyptus citriodora HOOK.[J];JOURNAL OF SOUTH CHINA AGRICULTURAL UNIVERSITY;1996-02
8 Gu Wenxiang Duan Shunshan Luo Shiming (Institute of Tropical and Subtropical Ecology, South China Agric. Univ.,Guangzhou,510642);ECOLOGICAL CHARACTERISTIC OF TERPENOIDS AND THEIR ALLELOPATHIC EFFECTS TO PLANTS[J];JOURNAL OF SOUTH CHINA AGRICULTURAL UNIVERSITY;1998-04
9 YE Ju xin,TAO Yong,GE Gang,LUO li ping (Department of Biological Science and Technology Nanchang University Nanchang Jiangxi 330047);Exploitation and Utilization on Chemical Eco-information of Plants[J];ENVIRONMENT AND EXPLOITATION;2000-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved