Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Plant Science Journal》 2013-03
Add to Favorite Get Latest Update

Cloning and Expression Analysis of Cinnamyl Alcohol Dehydrogenase(SmCAD) Gene in Salvia miltiorrhiza Bunge

GE Qian,KUANG Jing,WU Yu-Cui,ZHANG Yuan,XIAO Ya-Ping,WANG Zhe-Zhi(Key Laboratory of Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry,National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China,College of Life Sciences,Shaanxi Normal University,Xi'an 710062,China)  
Cinnamyl alcohol dehydrogenase(CAD) catalyzes the reduction reaction of cinnamic aldehyde and its derivatives depending on the NADPH.It is the key enzyme in the last step of lignin biosynthesis.A novel CAD gene designated as SmCAD(GenBank accession No.: HQ162287) was cloned from Salvia miltiorrhiza Bunge according to the transcription databases.It contained a 1083 bp open reading frame,including 3 introns and 4 exons,and encoded a protein of 360 amino acids.The predicted SmCAD protein had Zn1 and Zn2 binding sites and NADP(H) binding domain.A total of 1202 bp promoter sequences were obtained using BD walking method,and sequence analysis revealed that there were MeJA,ABA,GA3 response elements and MYB binding sites.Real-time quantitative PCR showed that SmCAD was expressed in different organs,and its expression was induced by MeJA and inhibited by GA3.These results suggested that this gene may be involved in the response of exogenous signals.This study contributes to the theoretical basis of the specific function of SmCAD.
【Fund】: 陕西省自然科学基础研究计划项目(2012JQ4013);; 中央高校基本科研业务费专项资助资金(GK201102017);; 陕西省教育厅科学技术研究发展计划(12JK0833)
【CateGory Index】: S567.53
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 3 Hits
1 NI Zhi-yong,LI Bo,LU Meng,WANG Juan,BAI Yan,FAN Ling(Institute of Nuclear and Biological Technologies,Xinjiang Academy of Agriculture Sciences,Urumqi 830091,China);Cloning and Analyzing of Cinnamyl Alcohol Dehydrogenase Gene from Gossypium hirsuturm L.[J];Acta Agriculturae Boreali-Sinica;2010-02
2 HUANG Hai-yan,WANG Yu-fu,XUE Zhao-dong,QIU Cai-sheng,HAO Dong-mei(Institute of Bast Fiber Crops,Chinese Academy of Agricultural Sciencrs,Changsha 410205,China);Clone of Partial Sequence of Cinnamyl Alcohol Dehydrogenase Gene in Flax[J];Hubei Agricultural Sciences;2008-05
3 Gong YanXu Mengqiu(College of Life Sciences and Biotechnology,Beijing Forestry University,Beijing 100083);Advance in Cinnamyl Alcohol Dehydrogenase as a Key Enzyme of Lignin Biosynthesis[J];Biotechnology Bulletin;2010-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 NI Zhi-yong1,2,Lü Meng1,LI Bo1,Lü Shu-ping1,FAN Ling1(1 Institute of Nuclear and Biological Technologies,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China;2 Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China);Structure and Expression of GhCCR1 from Gossypium hirsutum L.[J];Acta Botanica Boreali-Occidentalia Sinica;2011-01
2 LI Bo,NI Zhi-yong,FAN Ling(Institute of Nuclear and Biological Technologies,Xinjiang Academy of Agriculture Sciences,Urumqi 830091,China);Prokaryotic Expression,Purification and Identification of Caffeic acid-3-O-methyltransferase Gene from Gossypium hirsuturm L.[J];Acta Agriculturae Boreali-Sinica;2010-S1
3 NI Zhi-yong1,2,LU Shu-ping1,LI Bo1,FAN Ling1(1.Institute of Nuclear and Biological Technologies,Xinjiang Academy of Agricultural Sciences,Urumqi830091,China;2.Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China);Structural and Expression Analysis of GhCAD1 Gene from Gossypium hirsuturm L.[J];Acta Agriculturae Boreali-Sinica;2011-02
4 SHE Chao-wen1,2,3, XU Dong1, ZHANH Qing-hua1, JIANG Xiang-hui1,2,3(1.Department of Life Science; 2.Key Laboratory of Hunan Province for Study and Utilization of Ethnic Medicinal Plant Resources;3.Key Laboratory of Hunan Higher Education for Hunan-western Medicinal Plant and Ethnobotany,Huaihua University,Huaihua,Hunan 418008);Bioinformatics analysis of CAD gene from Cunninghamia lanceolata(Lamb.)Hook[J];Journal of Huaihua University;2009-11
5 Wang Song Chen Zhiqiang Tan Zhijian He Junming Guangzhou Hospital of Traditional Chinese Medicine,Guangzhou,Guangdong,China 510120;Clinical Study on the Methods of Promoting Blood Flow,Replenishing Qi and Clearing Heat in Healing Wound Tissue[J];China Journal of Chinese Medicine;2012-08
6 YU Yue-hua,GENG Hong-wei,CHEN Quan-jia,GAO Wen-wei,WANG Li-ping,QU Yan-ying(College of Agronomy,Xinjiang Agricultural University,Key Laboratory of Agricultural Biological Technology, Xinjiang Agricultural University,Urumqi 830052,China);Prokaryotic Expression Analysis of Transcription Factor TaSIM from Triticum aestivum L.[J];Acta Agriculturae Boreali-Sinica;2013-02
7 ZHAN Yiyin1,MA Jin2(1.Liaoning University of Traditional Chinese Medicine,Shenyang 110847,Liaoning,China;2.Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110032,Liaoning,China);Application of Yushenlu in Treatment of Chronic Glomerulonephritis[J];Journal of Liaoning University of Traditional Chinese Medicine;2013-05
8 YANG Yi-yong,LU Jing (Dept.of Traditional Chinese Medicine,Tongji Hospital,Tongji university,Shanghai 200065,China);Effect of prescription of YangYinHuashiYinReJieDu on chronic hepatitis B[J];Journal of Tongji University(Medical Science);2011-06
9 ZHAO Dan1,LI Xiao-Yu3,CHEN Jian3,ZHAO De-Gang1,2(1.Guizhou Key Labrotary of Agricultural Bioengineering,Guizhou University,Guizhou Guiyang 550025,China;2.College of Life Sciences,Guizhou University,Guizhou Guiyang 550025,China;3.Biological and Environmental Sciences,Huangshan University,Anhui Huangshan 242700,China;4.Department of Agriculture Bureau of Sihong County,Jiangsu Sihong 223900,China);Cloning and Sequence Analysis of the Full-length cDNA of Cinnamyl Alcohol Dehydrogenase Gene from Eucommia ulmoides Olive[J];Journal of Mountain Agriculture and Biology;2012-04
10 ZHANG Zhi-Xing 1,2,LI Zhong 1,2,CHEN Jun 1,2,LI Qi-Song 1,2,CHEN Long-Huai 1,2,CHEN Hong-Fei 1,2,HUANG Jin-Wen 1,and LIN Wen-Xiong 1,2,1 Institute of Agricultural Ecology,Fujian Agricultural and Forestry University,Fuzhou 350002,China;2 School of Life Sciences,Fujian Agricultural and Forestry University,Fuzhou 35002,China;Effects of Nitrogen Management on Protein Expression of Flag Leaves during Grain-filling Period in Large Panicle Rice (Oryza sativa L.)[J];Acta Agronomica Sinica;2011-05
【Secondary Citations】
Chinese Journal Full-text Database 9 Hits
1 WANG Wen-sheng,WANG Xing-fen,MA Zhi-ying,ZHANG Gui-yin(Key Laboratory of Crop Germplasm Resources of Hebei Province,Agricultural University of Hebei,Baoding 071001,China);Identification of BAC Clones Related with Verticillium Wilt Resistance Gene and Construction of Sub-clone Library[J];Acta Agriculturae Boreali-Sinica;2006-S1
2 NI Zhi-yong,MA Wen-jing,LU Meng,WANG Juan,LI Bo,FAN Ling(Institute of Nuclear and Biological Technologies,Xinjiang Academy of Agriculture Sciences,Urumqi 830091,China);Molecular Cloning of GhCAD6 Gene and Construction of Its Sense,Anti-sense and RNAi Expression Vectors[J];Acta Agriculturae Boreali-Sinica;2009-06
3 LIU Bao-ye~(1,2) Liang Guo-lu~2 ZHANG Zeng-yan~(1) (1.National Key Science Facility of Crop Gene Resources and Gene Improvement,Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100081;2.College of Horticulture and Landscape Architecture,Southwest University,Chongqing 400716);PROKARYOTIC EXPRESSION,REFOLDING AND ANTIFUNGAL ACTIVITY OF A RICE CHITINASE in vitro[J];Journal of Nuclear Agricultural Sciences;2009-03
4 WU Yaoting , ZHANG Hengmu, LIU Jinyuan(Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China);Cellulose Biosynthesis in Developing Cotton Fibers[J];Acta Gossypii Sinica;2003-03
5 NI Zhi-yong1,2,XU Zhao-shi2,LIU Li2,LI Lian-cheng2,CHAI Yan1,CHEN Ming2,MA You-zhi2(1.College of Agronomy,Northwest A & F University,Yangling,Shaanxi 712100,China;2.National Key Facility for Crop Gene Resources and Genetic Improvement(NFCRI),Key Laboratory of Crop Genetics and Breeding of Ministry of Agriculture,Institute of Crop Science,Chinese Academy of Agricultural Sciences(CAAS),Beijing 100081,China);Isolation and Characterization of a Transcription Factor TaDREB6 Gene from Triticum aestivum L.[J];Journal of Triticeae Crops;2008-03
6 Wang Shu-Fen, et al Department of Pathophysiology China Medical University, Chenyang;Effect of Tanshinone on human polymorphonuclear leucocyte chemokinesis[J];Chinese Journal of Pathophysiology;1986-02
7 Luo Houwei, Gao Jiwei~* and Zheng Jiarun~* (Department of Phytochemistry, ~*Institute of Dermatology Chinese Academy of Medical Sciences, Nanjing);RELATIONSHIP BETWEEN STRUCTURE AND ANTIBACTERIAL ACTIVITIES OF TANSHINONES AND RELATED COMPOUNDS[J];Journal of China Pharmaceutical University;1988-04
8 WEI Jian-Hua, SONG Yan-Ru (Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, China);Recent Advances in Study of Lignin Biosynthesis and Manipulation[J];Acta Botanica Sinica;2001-08
9 Ling Fan1, Wei-Jun Shi2, Wen-Ran Hu1, Xiao-Yan Hao1, Dong-Mei Wang1, Hui Yuan1 and Hong-Ying Yan1 (1Institute of Nuclear and Biological Technologies, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China; 2Institute of Economic Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China);Molecular and Biochemical Evidence for Phenylpropanoid Synthesis and Presence of Wall-linked Phenolics in Cotton Fibers[J];植物学报(英文版);2009-07
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved