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《Plant Science Journal》 2018-05
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Isolation,identification and characterization of reverse transcriptase sequence from Ty1-copia retrotransposon in Avena nuda

Guo Xiao-Fang;Jia Ju-Qing;Zhang Xiao-Jun;Zhang Chun-Lai;Zhang Mei-Jun;Feng Mei-Chen;Yang Wu-De;College of Agronomy,Shanxi Agricultural University;Institute of Crop Science,Shanxi Academy of Agricultural Science;  
Using degenerate oligonucleotide primers corresponding to the conserved domains of the Ty1-copia retrotransposon reverse transcriptase,23 Ty1-copia retrotransposons reverse transcriptase sequences were isolated by polymerase chain reaction( PCR) from the genome of Avena nuda L.,and characterization,phylogeny and transcriptional activities of these sequences were carried out. Results showed that the retrotransposons had high heterogeneity,with 45%-98% identity between sequences. The DNA sequences displayed mutations,including insertions, frameshifts, and stop codons, but the frequencies were not high.Phylogenetic analysis showed that the Ty1-copia retrotransposons of oats were mainly transmitted vertically during evolution. Five Ty1-copia retrotransposons with transcriptionalactivity were found by searching the oat gene expression database.
【Fund】: 国家自然科学基金项目(31101199);; 山西省应用基础研究计划项目(201601D102047 201601D102051);; 山西省重点研发计划重点项目(201703D211001-03-01);; 山西省农谷建设科研专项(SXNGJSKYZX201702);; 山西农业大学专业提升计划项目(TSJH1406)~~
【CateGory Index】: S512.6;Q943.2
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