USING IMPROVED FLUORESCENCE PROBE ASSAYING LYMPHOCYTE MEMBRANE FLUIDITY AND ITS CLINICAL USES
Geng Tao; Ma Aiqun, Liu Zhiquan(The Cardiovascular Medicine, First Affiliated Hospital)
In this paper, the lymphocyte membrane fluidity was abserved using improved fluorescence probe. This method did not need to seperatelymphocyte membrane, and it is simple and sensitive. The inter-assay CV was 0. 9%. Using thismethod, the lymphocyte membrane fluidity wasstudied in 20 health people and 41 patients withheart failure. The results showed that the lymphocvte membrane fluorescence polarization is 0. 259±0. 019 in health group and 0. 345±0. 021 in patientgroup. The latter increased significantly (P0. 05). The change of lymphocyte membrane fluiditywas the same as that of seperated erythrocyte membrane. There was positive correlation between thelymphocyte membrane fluidity and seperated erythrocyte membrane fluidity (r=0. 937, P0. 05).
【CateGory Index】： R541.61