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《Journal of Xi'an Medical University(Chinese)》 2003-01
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Improvement of the technique of bipolar electrode implantation for gastrointestinal myoelectrical recordings in rats

Chai Ningli, Dong Lei, Du Kexin,Wang Jianhua,Yan Likun,Dong Xilin,Fang Ping (Department of Digestion,Second Hospital of Xi'an Jiaotong University,Xi'an 710004,China)  
Objective To improve the technique of bipolar electrode implantation for myoeletrical recordings in rats. Methods We chosed teflon-coated seven-strand silver thread wire with outer diameter of only 0.5?mm to make recording electrode, and used injection needles as a trocar to implant the electrode in the bowel wall. Results We implanted a total of 160 pairs of bipolar electrodes into the bowels of rats. About a week later, 154 pairs of electrodes (96.3%) continuously functioned allowing repeated gastrointestinal myoeletrical recordings. 123 electrodes(76.9%)worked well over 5 weeks. Conclusion Our technique was a great improvement over the old one and its advantages include: ①There is little technical difficulty and high success rate in implantation; ②There is little damage on the subjects and can be used in many small animals and humans; ③Many pairs of electrodes implantation and many places myoelectrical recordings can be made at the same time; ④It has long term durability, observation of gastrointestinal myoeletrical activity near physiological state continuously.
【Fund】: 国家自然科学基金资助项目 (No .30 170 414 )
【CateGory Index】: R741.044
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