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《Journal of Xi'an Jiaotong University(Medical Sciences)》 2005-01
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Cloning and identifying of complete major outer-membrane protein gene of chlamydia trachomatis serovar D

Yang Zhangmin, Han Junhong, Zheng Jin, Yang Xiaofeng, Lai Baochang, Wang Yili, Si Lüsheng (Insitute for Cancer Research, School of Life science & Technology, Xi'an Jiaotong University, Xi'an 710061, China)  
Objective To clone target gene MOMP from cultured cells infected with chlamydia trachomatis serovar D. Methods The genome DNA of chlamydia trachomatis serovar D was extracted from cell culture and subjected to PCR as template. The MOMP gene was amplified and recombined with plasmid pUCmT. The recombinant plasmid pUCmT/MOMP was identified by restrictive enzyme digestion, PCR amplification and sequencing. Results The complete MOMP gene was cloned successfully and inserted into pUCmT plamid. Conclusion The target gene cloned from chlamydia trachomatis serovar D might provide a ready source for vaccine preparation.
【CateGory Index】: R346
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【References】
Chinese Journal Full-text Database 1 Hits
1 WANG Xiao-hong,LUO Kai-jun,GUO Jing,HOU Juan,LI Hui (Medical College of Jishou University,Jishou 416000,Hunan,China);Study of chlamydial urogenital infection and Omp1 gene variants in women in Xiangxin,China[J];Journal of Pathogen Biology;2009-12
【Citations】
Chinese Journal Full-text Database 1 Hits
1 PENG Hui,NING Bo,FENG Lianqiang,ZHANG Chunyan,XU Lin (Department of Immunology,Sun Yat sen University of Medical Science,Guangzhou 510089);CONSTRUCTION OF AN EUKARYOTIC EXPRESSION VECTOR PCDNA3-MOMP CARRYING THE MAJOR OUTER MEMBRANE PROTEIN GENE FROM CHLAMYDIA TRACHOMATIS SEROVAR L2 AND PARTIAL SEQUENCE DETERMINATION[J];Chinese Journal of Zoonoses;2001-03
【Co-citations】
Chinese Journal Full-text Database 1 Hits
1 PENG Hui,NING Bo,YUANG Guangqing,XU Lin (Department of Immunology,Sun Yat sen University of Medical Sciences,GuangZhou 510089);Vaccinating mice with eukaryotic expression plasmid DNA coding MOMP from Chlamydia trachomatis serovar L2[J];Chinese Journal of Zoonoses;2003-01
【Co-references】
Chinese Journal Full-text Database 3 Hits
1 CHEN Li-li WU Yi-mou DENG Zhong-liang CAI Heng-ling YU Min-jun GU Wei-min ZHANG Jun-fang Institute of Pathogenic Biology,Nanhua University,Hengvang 421001.Hunan.China;Genotyping of Chlamydia trachomatis isolated from patients with sexually transmitted diseases from four cities in Southern China[J];Chinese Journal of Dermatology;2006-05
2 Shen Li; Wu Shixiao; Su Guofu;et al Childrne's hospital,Chonqqing University of Medical Sciences,Chongqing 620014 The MOMP gene VS4 DNA of Chlamydia trachomatis isolated from clinical samples were amplified by PCR.The recombinant plasmid containing the PC;CLONING AND SEQUENCING OF THE MOMP GENE VS4 DNA OF CHLAMYDIATRACHOMATIS AND THE APPLICATION IN THE STUDY OF VERTICAL TRANSMISSION[J];Chinese Journal of Microbiology and Immunology;1995-01
3 PENG Hui,NING Bo,FENG Lianqiang,ZHANG Chunyan,XU Lin (Department of Immunology,Sun Yat sen University of Medical Science,Guangzhou 510089);CONSTRUCTION OF AN EUKARYOTIC EXPRESSION VECTOR PCDNA3-MOMP CARRYING THE MAJOR OUTER MEMBRANE PROTEIN GENE FROM CHLAMYDIA TRACHOMATIS SEROVAR L2 AND PARTIAL SEQUENCE DETERMINATION[J];Chinese Journal of Zoonoses;2001-03
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Zhang Guanping, Ning Bo. Third Clinical College, Sun Yat sen University of Medical Sciences, Guangzhou 510630;PCR detection of chlamydia pnumoniae DNA in nasopharyngolaryngeal swab samples from patients with rhinitis and pharyngolaryngitis[J];CHINESE JOURNAL OF OTORHINOLARYNGOLOGY;1997-01
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