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《Journal of Xi'an Jiaotong University(Medical Sciences)》 2008-01
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Genetic polymorphism of DXS6799 locus in three minority populations from Yunnan province

Li Zhengkun,Li Shengbin(The National Laboratory of the Ministry of Health for Forensic Sciences,Medical School ofXi'an Jiaotong University;Key Laboratory of Environment and Genes Related to Diseases,Ministry of Education,Medical School of Xi'an Jiaotong University;Department ofForensic Sciences,Medical School of Xi'an Jiaotong University,Xi'an 710061,China)  
Objective To resolve the problem of the accuracy and standardization of short tandem repeat-polymerase chain reaction(STR-PCR) typing in forensic practice,the molecular clone technology has been used in producing the standard allelic ladders of DXS6799 locus.And the standard allelic ladder was applied in studying the Lisu,Pumi and De-ang populations in Yunnan Province,China.Methods Polymorphism of DXS6799 was analyzed by PCR and PAGE.Molecular cloning technology was employed to construct standard DXS6799 allelic ladder used for DXS6799 genotyping.Results The large quantities of standard allelic ladder of the locus were harvested,and the genetic polymorphisms of DXS6799 locus in three populations were studied.Conclusion The method is of high value for forensic DNA typing to construct standard ladders.DXS6799 is robust for genetic research and forensic application.
【CateGory Index】: R694
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