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《Journal of Xi'an Jiaotong University(Medical Sciences)》 2008-01
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Protective effect of 17β-estrodiol on retina neuron cell apoptosis induced by H_2O_2 by the method of FACS

Jia Guorong,Yu Xiaorui,Li Hongbo,Wang Shuhong,Han Yan(Department of Genetics and Molecular Biology,Medical School of Xi'an Jiaotong University,Xi'an 710061,China)  
Objective To observe H2O2induced neuronal cell apoptosis and neuroprotection of 17βE2 by the technique of FACS.Methods The primary retina neuronal cells of neonatal SD rats which were born in 24 h were cultured.After 4-5 days,we treated the cells using different factors,then observed the cell viability by MTT method and the cell apoptosis by FACS method,respectively.Results H2O2 treatment(200μmol/L) could effectively induce the cultured primary retina neuron cell apoptosis.The low concentration of H2O2 could not affect the cells,but the high concentration treatment could induce cell apoptosis,at the same time leading to normal cell decrease sharply;βE2 treatment(10 μmol/L) showed neuroprotection on the primary cultured retina neuron cells,but the low concentration of βE2 had no effects,and the high concentration of βE2 manifested toxic action on the cells.Conclusion The limited concentration of βE2 shows effects of anti-apoptosis and neuroprotection on the cultured primary retina cells.
【Fund】: 国家自然科学基金资助项目(No.30672286);; 陕西省自然科学基金资助项目(No.2005C261)
【CateGory Index】: R774.1
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