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《Journal of Xi'an Jiaotong University(Medical Sciences)》 2008-01
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The construction of hbmp7 recombinant adenovirus vector

Li Xinyou,Wang Jintang,Liu Miao,Wang Quanying,Yang Guangxiao,Li Meng(Department of Orthopedic,the First Affiliated Hospital,Medical School of Xi'an Jiaotong University,Xi'an 710061,China)  
Objective In order to investigate the treatment of bone defect repairing by recombinant adenovirus transudation the hBMP7 gene,the vector of hPMP7 recombinant Ad was constructed.Methods The gene of hBMP7 was inserted into the replicated defective adenovirus shuttle plasmid pACCMV-plpA.We constructed the vector of hBMP7 recombinant Ad.The recombinant Ad viral stock was packaged.Totally 293 cells were co-transfected with the rAd vector of plasmid pACCMV/rhBMP7 and packaging plasmid PJM17 by calcium phosphate precipitation.Results The recombinant viral vector of plasmid pACCMV/rhBMP7 was constructed successfully.We got the Ad pACCMV/rhBMP7 stocks of high tire 1×1011 pfu/mL.Conclusion We prepared the viral stock of rAd-BMP7 that can serve as the experimental study of gene therapy of bone defect repairing.
【CateGory Index】: Q78
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