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《Journal of Xi'an Jiaotong University(Medical Sciences)》 2010-01
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Effect of quercetin on the scar formation after the alkal cauterization in rat's cornea

LIU Yan-fang1,2,QIN Li1,ZHANG Da3,HAN Cai-juan4(1.Department of Ophthalmology,the First Affiliated Hospital,Medical School ofXi'an Jiaotong University,Xi'an 710061;2.Xi'an Children's Hospital,Xi'an 710003;3.Department of Surgery,Xi'an Central Hospital,Xi'an 710003;4.Division of Medical Affairs,Xi'an Health Bureau,Xi'an 710002,China)  
Objective To explore the possible effect and mechanism of quercetin(QU) in inhibiting scar formation after the alkali burn of rat's cornea.Methods We established corneal alkali-burn model on right eyes of the SD rats.The rats were divided into five groups randomly.The control group received blank ophthalmic gel;the QU treatment groups received 2.5,5,10,or 20g/kg quercetin ophthalmic gel,respectively.The rats were checked by slit-lamp microscope every day for the degree of corneal opacity,then were killed on day 4,7,14,21 and 28 after operation.The infiltration of the inflammatory cells was observed by histology,the arrangement and proliferation of the collagen fibers in the corneal stroma were observed by Masson trichrome staining.The expression of transforming growth factor-β1(TGF-β1) and receptor of transforming growth factor-βⅠ(TGF-βRⅠ) in cornea was observed by immunohistochemical method.Results The corneal opacity was less severe in the QU treatment groups than in the control group(P0.05).Corneal scar was inhibited better in 10g/kg QU and 20g/kg QU groups than in 2.5g/kg and 5g/kg QU groups,and 10g/kg and 20g/kg QU groups had no significant differences(P0.05).HE and Masson staining showed that the density of corneal stroma collagen fibers in the QU treatment groups was lower than that in the control group.The expression of TGF-β1 and TGF-βRⅠ reached the peak on day 7 after alkali burn,and then decreased slowly,which was close to the normal level.The expression of TGF-β1 and TGF-βRⅠ was inhibited better in 10g/kg and 20g/kg QU groups than in 2.5g/kg and 5g/kg groups,and 10g/kg and 20g/kg QU groups had no significant differences(P0.05).Conclusion QU ophthalmic gel canreduce formation of corneal scar to a certain extent,10g/kg is the optimal concentration.QU may play its role by inhibiting the expression of TGF-β1 and TGF-βRⅠ.
【CateGory Index】: R779.1
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 MAO Yong-qiu, LIU Xiao-jing, JIANG Yu, WU Hong-bing. Key Laboratory of Biotherapy of Human Diseases, Ministry of Education, P.R. China, Cancer Center, West China Hospital, Sichuan University, Chengdu 610041, China;Effect of Quercetin on the Signal Pathway of TGFβ1 in Activated Hepatic Stellate Cells[J];Journal of Sichuan University (Medical Sciences Edition);2004-06
【Co-citations】
Chinese Journal Full-text Database 1 Hits
1 XU Biao,PENG Ding-feng,HU Yong-jun (Puai Hospital of Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430035,China);Effect of quercetin combined with Praziquantel on expression of myocardial immediate early gene and transforming growth factor-β1 in mice infected with Schistosomiasis japanica[J];Chinese Traditional and Herbal Drugs;2009-11
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Yu Chunzhi, et al. Department of Ophthalmology, Xijing Hospital;Clinical Observations on the Treatment of Cornea! Scar with Huayunning[J];Journal of The Fourth Military Medical University;1988-01
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