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《Journal of Xi'an Jiaotong University(Medical Sciences)》 2015-01
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Significance of the quantitative analysis of Lactobacillus and Peptostreptococcus productus of gut microbiota in patients with type 2 diabetes

WU Xiao-kang;MA Chao-feng;YU Peng-bo;WANG Xiang-ling;HAN Lei;LI Miao-xian;XU Ji-ru;Department of Clinical Laboratory,the Second Affiliated Hospital,Medical School of Xi'an Jiaotong University;Center for Disease Control and Prevention of Xi'an;Center for Disease Control and Prevention of Shaanxi Province;Department of Immunology and Pathogenic Biology,Medical School of Xi'an Jiaotong University;  
Objective To analyze quantitatively the intestinal Lactobacillus genus and Peptostreptococcus productus of patients with type 2 diabetes and healthy people to compare the differences of intestinal flora between the two groups of people.Methods The total DNA of intestinal microbiota was extracted.The specific primers of bacterial genus were used for real-time fluorescent quantitative PCR amplification.And bacterial standard and standard curve were made.Then we analyzed quantitatively the content of two predominant bacterial genus in patients with type 2 diabetes and healthy people.Results Compared with that in the healthy controls,the number of the intestinal Peptostreptococcus productus in the patients with type 2 diabetes was significantly reduced.However,as for the number of intestinal Lactobacillus genus,no significant differences were found between the two groups.Conclusion Real-time PCR can be taken as an accurate and sensitive testing method to study the intestinal microbiota.The changes of the intestinal predominant bacterial genus in patients with diabetes suggest a certain correlation of the intestinal predominant bacterial genus with the occurrence and development of diabetes.
【Fund】: 中央高校基本科研业务费专项资金资助~~
【CateGory Index】: R587.1
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