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《Journal of Xi'an Jiaotong University(Medical Sciences)》 2017-02
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The role of SPHK-1 in non-small cell lung cancer drug-resistant cell line H460

GAO Ying;TIAN Mei-li;SONG Li-ping;Department of Radiotherapy Oncology,the First Affiliated Hospital of Xi'an Jiaotong University;  
Objective To examine the role of SPHK-1/S1 P and NFκB p65 signal pathway in non-small cell lung cancer(NSCLC)drug-resistant cells.Methods The drug-resistant cell line of lung cancer H460/DDP was constructed and its biological characteristics were identified successfully.The expression of SPHK-l was tested by RT-PCR and Western blot methods.S1 P and some proteins related to NFκB pathway were studied by Western blot.Results The drug-resistant lung cancer cell line H460/DDP was constructed and its drug-resistant ability was evaluated(IC50H460/DDP= 50.62μg/mL,RIH460/DDP = 2.95).Cisplatin at a concentration of 10-80 μg/mL significantly decreased cell death of drug-resistant cell line(P0.01).Western blot assay analysis showed that overexpressions of SPHK-l,S1 P and NFκB p65 were significantly higher in drug-resistant cell line than in their parent cell line H460(P=0.041 5,P=0.046 5,P=0.021 8).RT-PCR method revealed that SPHK-1 mRNA was overexpressed in drug-resistant cell line compared with that in their parent cell line H460(P0.05).More NFκB p65 protein in cell nucleus was expressed in drug-resistant cells than in parent cells.Conclusion SPHK-1/S1 P and NFκB p65 signal pathway may play an improtant role in the drug-resistant H460 cell line in non-small cell lung cancer.
【Fund】: 国家自然科学基金资助项目(No.81301937)~~
【CateGory Index】: R734.2
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 Yue-Yuan Zhong,Jie-An Huang,Shi-Quan Liu,MengBin Qin,Hui Jin,Department of Gastroenterology,the First Aff iliated Hospital of Guangxi Medical University,Nanning 530021,Guangxi Zhuang Autonomous Region,China;Role of Sphk1 in the proliferation and invasion of human colon cancer Lovo cells[J];世界华人消化杂志;2010-24
【Co-citations】
Chinese Journal Full-text Database 5 Hits
1 Liu Jiali;Liu Xiaohui;Department of Nephrology, Affiliated Hospital of North Sichuan Medical College;;Sphingosine kinases pathway[J];中华临床医师杂志(电子版);2016-06
2 WANG Ya-nan;XU Hong-yan;LI Xiang-ke;ZHANG Yan-yan;FAN Qing-xia;Oncology Department,the First Affiliated Hospital of Zhengzhou University;;Effects of sphingosine kinase 1 inhibitors in combination with 5-FU on gastric cancer MGC-803 cells and the possible mechanisms[J];西安交通大学学报(医学版);2014-03
3 SU Ying-jie,HUANG Jie-an,LIU Shi-quan,HUANG Juan-xiu,ZHONG Yue-yuan,TANG Guo-du,JIANG Hai-xing.Department of Gastroenterology,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China;The expression and clinical significance of SphK1 and nuclear factor-kB p65 in human colon carcinoma[J];中华内科杂志;2012-03
4 Ying-Jie Su,Jie-An Huang,Shi-Quan Liu,Yue-Yuan Zhong,Meng-Bin Qin,Department of Gastroenterology,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,Guangxi Zhuang Autonomous Region,China;Sphingosine kinase 1 enhances cell proliferation and migration and suppresses apoptosis in human colon cancer cell line lovo[J];世界华人消化杂志;2012-04
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