Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Xi'an Jiaotong University(Medical Sciences)》 2017-02
Add to Favorite Get Latest Update

Effect of TTF-1 promoter regulated miR-7 expression on the apoptosis of human lung cancer 95D cells in vitro

LU Jia;CHEN Chao;LIAO Zhen-yuan;CUI Pan-pan;LEI Liang-yu;XU Hua-lin;TIAN Dan;ZHENG Jing;XU Lin;Department of Immunology,Zunyi Medical College;Guizhou Provincial College-based Key Lab for Tumor Prevention and Treatment with Distinctive Medicines;Biological Therapy Talents Base of Guizhou Province;  
Objective To investigate the effect of thyroid transcription factor-1(TTF-1)promoter regulated miR-7 expression on the apoptosis of human lung cancer 95 D cells in vitroand to explore its significance.Methods A eukaryotic expression vector of TTF-1 was detected by FCM,and the apoptosis of cells was analyzed by TUNEL assay.Furthermore,the expression levels of phos-Casp-3 and phos-Casp-9 protein in 95 D cells were detected by Western blot.Meanwhile,the expression of CGGBP1 and EGFR,twotarget molecules of miR-7,were examined by immunofluorescence assay confocal microscopy.Finally,the expression of phosphorylated Akt and promoter regulation miR-7(p-TmiR-7)and control vector p-Cont which had been previous constructed were transiently transfected into the human lung cancer 95 D cells in vitro,respectively.TTF-1 promoter regulated miR-7 eukaryotic expression vector(named p-TmiR-7)and control vector p-Cont were transiently transfected into human lung cancer95 D cells in vitro,respectively.The expressions of phosphorylated Akt and Erk protein in each group were assayed by Western blot.Results At 48 h after transfection in vitro,the apoptosis ratio of cells in p-T-miR-7 transfection group significantly increased compared with that in p-Cont transfection group(P 0.05). Meanwhile,the expression levels of phos-Casp-3 and phos Casp-9 protein significantly increased(P0.05).Furthermore,the expressions of EGFR and CGGBP1 protein decreased obviously,accompanied by decreased expressions of phos-Akt and phos-Erk in p-T-miR-7 transfection group(P 0.05).Conclusion TTF-1 promoter regulated miR-7expression can induce the apoptosis of human lung cancer cells in vitro,which provides some experimental basis for the development of human lung cancer gene targeting therapeutic strategy.
【Fund】: 国家自然科学基金资助项目(No.31370918);; 贵州省高层次人才项目(黔科合人才(2016)4031号);; 贵州省教育厅特色重点实验室建设项目(黔教合KY字[2014]212)~~
【CateGory Index】: R734.2
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 4 Hits
1 Chen Chao;Zhao Juanjuan;Hu Yan;Guo Mengmeng;Tao Yijing;Zhou Ya;Qin Nalin;Zheng Jing;Xu Lin;Department of Immunology,Zunyi Medical University;Department of Medical Physics,Zunyi Medical University;;Construction of eukaryotic expression vector TTF-1 promoter regulation microRNA-7[J];遵义医学院学报;2014-03
2 HU Yan;LIAO Zhenyuan;CHEN Chao;QIN Nalin;ZHENG Jing;TIAN Dan;LI Yongju;ZHU Shunfei;LUO Junmin;XU Lin;Department of Immunology,Zunyi Medical College,Immunology Graduate Students Education Innovation Base in Guizhou Province;;Over-expressed microRNA-7 inhibits the growth of human lung cancer cells via suppressing CGGBP1 expression[J];细胞与分子免疫学杂志;2014-02
3 XU Lin1,REN Tao2,QING An-dong1,ZHOU Ya3,Luo Jun-ming1,Yao Xin-sheng1,LI Ying1 (1.Department of Immunology,Zunyi Medical College,Guizhou Zunyi 563003,China;2.Department of Respiratory Medicine,East Hospital,Tongji University,Shanghai 200120,China;3.Department of Medical Physics,Zunyi Medical College,Guizhou Zunyi 563003,China);The effects of microRNA-7 on the invasion of human lung cancer cell line 95D cells in vitro[J];遵义医学院学报;2011-01
4 XU Lin1,REN Tao2,ZHOU Ya3,QIN An-dong1,ZHENG Jing1(1.Department of Immunology,Zunyi Medical College,Zunyi 563003,China;2.Department of Respiratory Medicine,East Hospital,Tongji University,Shanghai 200120,China;3.Department of Medical Physics,Zunyi Medical College,Zunyi 563003,China);The effects of miRNA-7 on the proliferation of human lung cancer cell line 95D cells[J];肿瘤;2010-09
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Li Nan;Li Yunxia;Jia Hui;Lou Lin;Xia Shuyue;Department of Respiratory Medicine,Central Hospital affiliated to Shenyang Medical College;;Research advances on miR-155 in lung disease[J];国际呼吸杂志;2017-08
2 CHEN Yanyan;LIAO Jiao;ZHAO Yuxia;Department of Radiation Oncology of Cancer Centre,The Fouth Affiliated Hospital of China Medical University;;Progress in research on relationship between microRNA-7 and lung cancer[J];实用肿瘤学杂志;2016-01
3 Zhao Juanjuan;Xu Hualin;Tao Yijing;Guo Mengmeng;Zhou Ya;Chen Chao;Qin Nalin;Zheng Jing;Tian Dan;Xu Lin;Department of Immunology,Zunyi Medical University,Talent Base of Biological Therapy of Guizhou Province;Guizhou Provincial College-based Key Lab for Tumor Prevention and Treatment with Distinctive Medicines,Zunyi Medical University;Department of Medical Physics,Zunyi Medical University;;Stem-loop real-time PCR assay for quantitative detection of miRNA-7 by specific primers using SYBR Green Ⅰ[J];遵义医学院学报;2015-06
4 Yang Yarui;He Yun;Gong Chunmei;Molecular Biology Lab,Shenzhen Center for Chronic Disease Control;School of Public Health,Sun Yat-sen University;;Research progress of microRNA and lung cancer pathogenesis[J];现代肿瘤医学;2015-22
5 TAO Yi-Jing;ZHU Shun-Fei;CHEN Chao;ZHAO Juan-Juan;GUO Meng-Meng;ZHANG Yi-Xiong;QIN Na-Lin;XU Lin;Department of Immunology and Immunology Innovation Base of Postgraduate Education,Zunyi Medical College;;Influence of microRNA-7 knock down on development of CD4~+SP cells in murinethymus[J];中国免疫学杂志;2015-09
6 ZHAO Juan-Juan;ZHU Shun-Fei;XU Hua-Lin;HU Yan;GUO Meng-Meng;TAO Yi-Jing;ZHOU Ya;QIN Na-Lin;ZHENG Jing;XU Lin;Department of Immunology and Immunology Innovation Base of Postgraduate Education,Zunyi Medical College;;Effect of microRNA-7 on αβT lymphocytes of mouse mesenteric lymph nodes[J];中国免疫学杂志;2015-09
7 Zeng Chunyan;Zhan Yishan;Huang Jun;Chen Jiang;Lyu Nonghua;Chen Youxiang;Department of Oncology,The First Affiliated Hospital of Nanchang University;;Clinical significance of microRNA-7 expression and its correlation with focal adhesion kinase in colorectal cancer[J];中华消化杂志;2015-08
8 Guo Mengmeng;Liao Zhenyuan;Zhao Juanjuan;Tao Yijing;Hu Yan;Chen Chao;Qin Nalin;Zheng Jing;Xu Lin;Department of Immunology,Immunology Graduate Education and Innovation Base of Guizhou Province,Zunyi Medical University;;Construction of an eukaryotic expression vector encoding miRNA-7 with self promoter and its significance[J];遵义医学院学报;2015-03
9 GONG Xing;Xiangyang Hospital,Hubei University of Medicine;;Research about the Change of miR in Serum and Tissues of Lung Cancer[J];实用癌症杂志;2015-05
10 CHEN Chao;LIAO Zhenyuan;LI Ying;LI Yongju;LUO Junmin;XU Lin;Department of Immunonology,Zunyi Medical University;;Effect of micro RNA-7 Overexpression on Apoptosis of Human Lung Cancer Cell Line 95D[J];肿瘤防治研究;2015-02
【Secondary Citations】
Chinese Journal Full-text Database 9 Hits
1 HU Yan;LIAO Zhenyuan;CHEN Chao;QIN Nalin;ZHENG Jing;TIAN Dan;LI Yongju;ZHU Shunfei;LUO Junmin;XU Lin;Department of Immunology,Zunyi Medical College,Immunology Graduate Students Education Innovation Base in Guizhou Province;;Over-expressed microRNA-7 inhibits the growth of human lung cancer cells via suppressing CGGBP1 expression[J];细胞与分子免疫学杂志;2014-02
2 Zhu shunfei;Liao zhenyuan;Hu yan;Chen chao;Li yongju;Liu sijing;Luo junmin;Xiong sidong;Xu lin;Department of Immunology and Immunology Innovation Base of Postgraduate Education Department,Zunyi Medical University;Zunyi Medical University;;The effects of microRNA-7 on the metastasis of human lung cancer 95D cells in vitro and in vivo[J];遵义医学院学报;2013-05
3 SONG Yong-xiang1,2,LI Ying1,3,QIN An-dong1,3,LIAO Zhen-yuan1,3, LI Yong-ju1,3,LUO Jun-min1,3,REN Tao4,XU Lin1,3(1.Department of Immunology,Zunyi Medical College,Zunyi 563000; 2.Cardiothoracic Surgery,the Affiliated Hospital of Zunyi Medical College,Zunyi 563000; 3.Innovation Base of Immunological Graduate Education,Zunyi 563000; 4.East Hospital,Tongji University,Shanghai 200120,China);Construction and identification of eukaryotic expression vector encoding miRNA-7[J];西安交通大学学报(医学版);2013-04
4 Lan SHAO 1,2 , Zhengbo SONG 1,2 , Yiping ZHANG 1,2 , Dan SU 2,3 1 Department of Medical Oncology, Zhejiang Cancer Hospital, Hangzhou 310022, China; 2 Key Laboratory Diagnosis and Treatment Technology on Thoracic Oncology, Hangzhou 310022, China; 3 Zhejiang Cancer Research Institute, Zhejiang Cancer Hospital, Hangzhou 310022, China;Advances of Molecular Subtype and Targeted Therapy of Lung Cancer[J];中国肺癌杂志;2012-09
5 Zheng jing,Li ying,Qin andong,Qin nalin,Luo junmin,Xu lin (Department of Immunology and Immunology Innovation Base of Postgraduate Education,Zunyi Medical College,Guizhou Zunyi 563099,China);The expression of miRNA-7 in different murine organs and its significance[J];遵义医学院学报;2012-02
6 XU Lin1,REN Tao2,QING An-dong1,ZHOU Ya3,Luo Jun-ming1,Yao Xin-sheng1,LI Ying1 (1.Department of Immunology,Zunyi Medical College,Guizhou Zunyi 563003,China;2.Department of Respiratory Medicine,East Hospital,Tongji University,Shanghai 200120,China;3.Department of Medical Physics,Zunyi Medical College,Guizhou Zunyi 563003,China);The effects of microRNA-7 on the invasion of human lung cancer cell line 95D cells in vitro[J];遵义医学院学报;2011-01
7 XU Lin1,REN Tao2,ZHOU Ya3,QIN An-dong1,ZHENG Jing1(1.Department of Immunology,Zunyi Medical College,Zunyi 563003,China;2.Department of Respiratory Medicine,East Hospital,Tongji University,Shanghai 200120,China;3.Department of Medical Physics,Zunyi Medical College,Zunyi 563003,China);The effects of miRNA-7 on the proliferation of human lung cancer cell line 95D cells[J];肿瘤;2010-09
8 LI Zi-ming1, CHEN Zhi-wei1, NIU Xiao-min1,LU Shun1, LIAO Mei-lin1, ZHANG Jie2 (1. Shanghai Lung Cancer Center, Shanghai Chest Hospital, Shanghai Jiaotong University, Shanghai 200030, China; 2. Department of Pathology, Shanghai Chest Hospital, Shanghai Jiaotong University, Shanghai 200030, China);Effects of anti-EGFR monoclonal antibody cetuximab in combination with the EGFR tyrosine kinase inhibitor gefitinib on apoptosis of human pulmonary adenocarcinoma cells and their action mechanism[J];肿瘤;2010-06
9 Jianfang QIAN~1;Aiqiang DONG~1;Minjian KONG~1;Zhiyuan MA~2;Junqiang FAN~1;and Guanyu JIANG~3 ~1Department of Cardiothoracic Surgery,Second Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou 310009,China;~2Department of Cardiovascular Surgery,Shanghai Jiaotong University Affiliated First People's Hospital,Shanghai 200080,China;~3Department of Emergency,Second Affiliated Hospital School of Medicine,Zhejiang University,Hangzhou 310009,China;Suppression of Type 1 Insulin-like Growth Factor Receptor Expression by Small Interfering RNA Inhibits A549 Human Lung Cancer Cell Invasion in vitro and Metastasis in Xenograft Nude Mice[J];Acta Biochimica et Biophysica Sinica;2007-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved