Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Xi'an Jiaotong University(Medical Sciences)》 2017-02
Add to Favorite Get Latest Update

Expressions of epithelial-mesenchymal transition-associated genes in cervical cancer cell lines

LI Xiao-feng;CHEN Wei;LI Xu;ZHAO Le;Department of Pathology,the First Affiliated Hospital of Xi'an Jiaotong University;Department of Laboratory Medicine,the First Affiliated Hospital of Xi'an Jiaotong University;Center for Translational Medicine,the First Affiliated Hospital of Xi'an Jiaotong University;  
Objective Epithelial-mesenchymal transition(EMT)is thought to be one of the important mechanisms of invasion and metastasis of carcinoma cells,which includes down-regulation of epithelial cell markers and up-regulation of mesenchymal markers.The aim of our study was to investigate whether EMT occurs in cervical cancer cell lines and determine the proper cell model to study the mechanism of EMT in cervical cancer.MethodsWe detected the mRNA expression of EMT-associated genes,namely,E-cadherin,vimentin,Snail and Slug,in four cervical cancer cell lines using reverse transcriptase-polymerase chain reaction analysis.E-cadherin and vimentin protein expression levels were examined using Western blotting.Snail and Slug protein expression levels were examined by immunocytochemical staining.Results Vimentin and Snail mRNA was expressed in all of the four cell lines.E-cadherin and Slug mRNA was expressed in SiHa,HeLa and RJC-1 cells except CS1213 cells.E-cadherin protein was found in SiHa and RJC-1 cells whereas vimentin protein was detected in HeLa and CS1213.Snail protein was expressed in all of the four cell lines.Slug protein was found in SiHa,HeLa and RJC-1 cells.Conclusion It seems that HeLa and CS1213 display the phenotype of mesenchymal cells whereas SiHa and RJC-1 remain the epithelial phenotype.
【Fund】: 国家自然科学基金资助项目(No.30872928)~~
【CateGory Index】: R737.33
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 1 Hits
1 FENG Ju;YUAN Zhongfu;QIU Haifeng;Department of Obstetrics and Gynecology, Central Hospital of Jiaozuo Coal Industry (Group) Co., Ltd.;;Reversal effect of RNA interference targeting FAK on the migration and invasion of cervical cancer cells[J];癌症进展;2018-02
【Citations】
Chinese Journal Full-text Database 2 Hits
1 Han Jin,Chen Wei,Li Xu (Center for Laboratory Medicine,the First Affiliated Hospital,Medical School of Xi'an Jiaotong University,Xi'an 710061,China);Fragile histidine triad transcription abnormalities and human papillomavirus16 infection in human cervical carcinoma[J];西安交通大学学报(医学版);2008-02
2 LI Xu *,CHEN Wei,YANG Yu-cong,SI Lü-sheng. *Center for Laboratory Medicine,First Hospital of Jiaotong University,Xi′an 710061,China;Establishment and characterization of a new squamous cell carcinoma cell line CS1213 from the human uterine cervix[J];中华妇产科杂志;2003-10
【Co-citations】
Chinese Journal Full-text Database 4 Hits
1 LI Xiao-feng;CHEN Wei;LI Xu;ZHAO Le;Department of Pathology,the First Affiliated Hospital of Xi'an Jiaotong University;Department of Laboratory Medicine,the First Affiliated Hospital of Xi'an Jiaotong University;Center for Translational Medicine,the First Affiliated Hospital of Xi'an Jiaotong University;;Expressions of epithelial-mesenchymal transition-associated genes in cervical cancer cell lines[J];西安交通大学学报(医学版);2017-02
2 Han Jin,Chen Wei,Li Xu (Center for Laboratory Medicine,the First Affiliated Hospital,Medical School of Xi'an Jiaotong University,Xi'an 710061,China);Fragile histidine triad transcription abnormalities and human papillomavirus16 infection in human cervical carcinoma[J];西安交通大学学报(医学版);2008-02
3 WANG Ying, LI Xu, CHEN Wei Medical Laboratory Center, First Hospital of Xi'an Jiaotong University, Xi'an 710061, China;Expression, structure prediction and functional analysis of murine single-chain fragment vari- able antibody against human cervical cancer[J];南方医科大学学报;2006-01
4 Chen Wei, Li Xu, Yang Yucong, Cheng Xiaoli (Center of Laboratory Medicine, First Hospital of Xi'an Jiaotong University, Xi 'an 710061, China);Study on gene expression profile of subclonal cell lines in two different anchorage-independent uterine cervical carcinoma[J];西安交通大学学报(医学版);2004-06
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 WANG Jing-wen;WANG Si-nian;SUN Suo-zhu;ZHANG Xin;YU Nan;LI Feng-sheng;Graduate School,Liaoning University of Traditional Chinese Medicine;Laboratory of Nuclear and Radiation Damage,The General Hospital of the PLA Rocket Force;Department of Pathology,The General Hospital of the PLA Rocket Force;Cancer Hospital of China Medical University,Liaoning Cancer Hospital and Institute;;The Effects of Inhibiting CXCR7 Gene Expression on Migration and Invasion of HeLa Cells and Expression of Cervical Cancer Tissues[J];国际妇产科学杂志;2017-02
2 Gao Ning;Zhang Jing;Du Juan;Mu Mingtao;Liu Junjun;Jin Wenjie;He Huimin;Hou Yingchun;Medical College of Yan'an University;College of Life Sciences, Shaanxi Normal University;;FAK silencing promotes the depolymerization of cytoskeleton proteins and morphological damage in SGC-7901 human gastric cancer cells[J];中国组织化学与细胞化学杂志;2017-01
3 Wang Xiangyu;Xu Jinquan;Sun Li;School of Medicine and Life Sciences,University of Jinan-Shandong Academy of Medical Sciences;Institute of Biomedical Sciences,National Sun Yatsen University;Departments of Gynecological Oncology,Shandong Cancer Hospital Affiliated Shandong University;;Research of Rsf-1/HBXAP expression and clinical pathological characteristics in cervical cancer[J];现代妇产科进展;2017-02
4 LI Xiao-feng;CHEN Wei;LI Xu;ZHAO Le;Department of Pathology,the First Affiliated Hospital of Xi'an Jiaotong University;Department of Laboratory Medicine,the First Affiliated Hospital of Xi'an Jiaotong University;Center for Translational Medicine,the First Affiliated Hospital of Xi'an Jiaotong University;;Expressions of epithelial-mesenchymal transition-associated genes in cervical cancer cell lines[J];西安交通大学学报(医学版);2017-02
5 HAO Zhenfeng;WANG Juan;ZHOU Liulin;LIU Jing;KONG Guimei;YANG Jishi;QIAN Jing;Taixing Peo Hosp;Coll of Med,Yangzhou Univ;;The expression and clinical significance of the FAK in cervical cancer[J];扬州大学学报(农业与生命科学版);2016-04
6 Li Jiyou;Jia Dong;Department of Emergency Surgery,the First Affiliated Hospital of Harbin Medical University;Cardiovascular Catheter Lab,the First Affiliated Hospital of Harbin Medical University;;Effects of focal adhesion kinase inhibition on apoptosis of human gastric carcinoma cells[J];现代肿瘤医学;2017-01
7 Chen Jinsong;Huang Jiongqiang;Dong Shihao;Huang Xiaohui;Department of Surgery,the First Affiliated Hospital of Guangzhou Medical University;;Effects of microRNA-379-5p on proliferation,migration and invasion of hepatocellular carcinoma cell line[J];中华医学杂志;2016-18
8 ZHUGE Chun-feng;LIU Shi-quan;TAN Lin;QIN Meng-bin;LIANG Meng-zi;HUANG Jie-an;Department of Gastroenterology,First Affiliated Hospital of Guangxi Medical University;;Effects of Sph K1 and FAK on epithelial-mesenchymal transition in colon cancer HCT116 cells[J];中国病理生理杂志;2016-03
9 CHEN Ying;WANG Dandan;ZHU Hong;HE Qiaojun;YANG Bo;Zhejiang Province Key Laboratory of Anti-Cancer Drug Research, College of Pharmaceutical Sciences, Zhejiang University;;Research and Development of Focal-Adhesion Kinase(FAK) and the Inhibitors[J];中国现代应用药学;2016-02
10 LI Miao,LIU Fang-Fang,DI Yuan-Yuan et al.Department of Gynecology and Obstetrics,the Second Affiliated Hospital of Guangdong Medical College,Zhanjiang 524003,Guangdong,China;Value of DNA quantitative analysis combined with colposcopy for diagnosis of cervical cancer and high-grade cervical intraepithelial neoplasia[J];中国妇幼保健;2012-18
【Secondary Citations】
Chinese Journal Full-text Database 5 Hits
1 ZHANG Wen-miao SHUAI Ci-xia ZHENG Fei-yun HUANG Yin-ping WANG Qun-ji ZHU-Hua HE Qiu-xiang The First Affiliated Hospital of Wenzhou Medical College,Wenzhou 325000,China;Expression of FHIT genes in CIN and cervical carcinoma and the relationship between FHIT gene and p53 and HPV16/18[J];中华肿瘤杂志;2006-06
2 Wu Ying Wang Shixuan Lu Yunping et alDepartment of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical College,Huazhong University of Science and Technology, Wuhan;The Significance of the Expression of FHIT in Cervical Carcinoma[J];中国肿瘤临床;2005-07
3 Shi Haiyan, Chen Xiaoduan (Department of Pathology, Affiliated Obstetrics and Gynecology Hospital, School of Medicine, Zhejiang University, Hangzhou 310006);Abnormal expression and loss of heterozygosity of the FHIT gene in cervical carcinomas[J];临床与实验病理学杂志;2004-01
4 LI Xu *,CHEN Wei,YANG Yu-cong,SI Lü-sheng. *Center for Laboratory Medicine,First Hospital of Jiaotong University,Xi′an 710061,China;Establishment and characterization of a new squamous cell carcinoma cell line CS1213 from the human uterine cervix[J];中华妇产科杂志;2003-10
5 Zhao Xingbo, Zhang Yidu, Su Yingkuan, et al. Shandong Provincal Hospital, Jinan 250021;Establishment and Characterization of a Xenografted Human Cervical Carcinoma in Nude Mice and Homologous Cell Line in Vitro[J];中华妇产科杂志;1997-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved