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《Journal of Xi'an Jiaotong University(Medical Sciences)》 2017-02
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miR-498 inhibits A549 cell migration and invasion by down-regulating FOXM1

TANG Xi;HU Ya;XU Yan-hua;WANG Chun-lin;QIU Ping;WANG Xiang-hui;Department of Oncology,Jingzhou Hospital of Tongji Medical College,Huazhong University of Science and Technology;Medical College,Yangtze University;Department of Cardiothoracic Surgery,Jingzhou Hospital of Tongji Medical College,Huazhong University of Science and Technology;  
Objective To verify whether miR-498 can inhibit A549 cell migration and invasion by downregulating FOXM1.Methods miR-498 mimic and miR-NC were transfected into A549 cells.Wound healing and Transwell method were employed to test the migratory ability and invasion ability of A549 cells;Western blot was used to detect the expressions of COL1A1,COL1A5 and FOXM1 in A549 cells.Luciferase assay was used to confirm whether FOXM1 is the target gene of miR-498.Results Compared with those in the control group,the expressions of COL1A1,COL1A5 and FOXM1 were decreased,and the migration and invasion abilities of A549 cells were decreased in the miR-498 group(both P0.01).The luciferase activity of the FOXM1-3′-UTR plasmid was significantly suppressed by miR-498(P0.05);over-expression of FOXM1 could reverse the effect of miR-498 on A549 cells.Conclusion miR-498 inhibits A549 cell migration and invasion by down-regulating FOXM1.
【Fund】: 湖北省教育厅项目(No.B2013280);; 湖北省卫生厅项目(No.2012Z-Y31)~~
【CateGory Index】: R734.2
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