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《Journal of Xi'an Jiaotong University(Medical Sciences)》 2017-02
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Differentiation of breast cancer stem cells into vascular endothelial cells and tumor angiogenesis

LI Jing;FU Yan;XIAO Guang-xiong;XU Jun;ZHANG Hao;Department of Thyroid Gland and Breast Surgery,the First People's Hospital of Jingmen City;Taihe Hospital of Shiyan City;the Affiliated Hospital of Hubei Medical College;  
Objective To explore the potential of breast cancer stem cells in differentiating into vascular endothelial cells and participating in the angiogenesis of breast tumor.Methods The expressions of mutant P53,CD31,VEGF and Her-2 amplification in tumor blood vessels were detected and the single cell suspension of breast cancer was prepared.CD44~+/CD24-/lowcells were selected and cultured in culture system.The expressions of CD31 and CD105 on endothelial cell surface were detected by EGM-2 endothelial cell culture medium.The ability of uptake of acetylated low-density lipoprotein was measured.The endothelial cells were cultured in vitro to observe their vascular structure.Results The expressions of CD31,VEGF and mutant P53 were found in paraffin sections of breast cancer tissue samples,and they were arranged along the vessel lumen or blood vessel sphere.Immunofluorescence showed that CD31 and DAPI signals were expressed intravascularly in breast cancer tissues.Four samples of Her-2 positive amplification and 9 cases of non-amplification were detected in the samples.The successful separation of breast cancer stem cells was carried out by immunomagnetic sorting.The percentage of CD44~+cells in the pre-sorting cells was(7.5±2.6)% and that of CD44~+cells was about(94.3±4.7)% after magnetic sorting(P0.05).The ratio of CD24~+cells was(48.2±9.4)% before sorting,and that of CD24~+cells was(4.3±1.6)% after immunomagnetic bead sorting.The proportion of CD105~+cells in mammary gland cells was(4.5±0.9)% and the proportion of CD31~+cells was(6.2±1.3)%.After cultured for three generations,the percentages of CD105~+cells and CD31~+cells were(79.6±9.3)% and(84.1±10.7)%,respectively(P0.05).DiL-Ac-LDL could be phagocytized by endothelial cells cultured in endothelial cell culture system.Vessel-like structure was found in the endothelial cell group while the control group did not have the tendency of vascular changes.Conclusion Breast cancer stem cell-derived endothelial cells differentiate into endothelial cells in vitro and have the ability of vascular formation,suggesting that breast cancer stem cells may be involved in the formation of tumor blood vessels.
【Fund】: 荆门市引导性科研项目资助(No.YDKY2016002)~~
【CateGory Index】: R737.9
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【Secondary Citations】
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1 YANG Yan,CHEN Qing-wei,CAO Guang-yu,LI Gui-qiong(Department of Gerontology,Second Affiliated Hospital of Chongqing Medical University,Chongqing 400010,China);Efficiency of rat bone marrow-derived endothelial progenitor cells on VEGF and SDF-1 expression with the aid of Tanshinone ⅡA[J];激光杂志;2012-02
2 ZHAI Xue-min1,HE Shui-xiang1,REN Mu-dan1,CHEN Jing-hong2,WANG Zhi-lun2,HAN Min1,HOU He-lei1(1.Department of Gastroenterology,The First Affiliated Hospital,College of Medicine,Xi'an Jiaotong University,Xi'an 710061,China; 2.Department of Public Health,College of Medicine,Xi'an Jiaotong University,Xi'an 710061,China);Effect of Tanshinone ⅡA on expression of EGF and EGFR in hepatocellular carcinoma cell line SMMC-7721[J];浙江大学学报(医学版);2009-02
3 JING Jing, ZHENG Hong, WANG Jing, LIN Ping, ZHANG Jie, XIONG Zhu-juan, WU Ya-ying, REN Jing-jing, YANG Hong-liang, WANG Xiu-jie△. Experimental Oncology Laboratory, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China;Growth Inhibition and Multidrug Resistance-reversing Effect of Tanshinone ⅡA on Human Breast Cancer Cell with Estrogen Receptor Negative[J];四川大学学报(医学版);2007-03
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